| 黄山贡菊组织培养与快速繁殖技术研究 |
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| 引用本文： | 陈黎,万志兵,曹玲玲.黄山贡菊组织培养与快速繁殖技术研究[J].黄山高等专科学校学报,2013(5):63-65. |
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| 作者姓名： | 陈黎 万志兵 曹玲玲 |
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| 作者单位： | 黄山学院生命与环境科学学院,安徽黄山245041 |
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| 基金项目： | 安徽省教育厅自然科学研究项目（KJ2011B169）;安徽省教育厅优秀青年人才基金项目（2012SQRL185） |
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| 摘 要： | 以黄山贡菊的茎尖、叶片及根为外植体,进行了黄山贡菊芽的诱导、继代增殖培养、瓶外生根壮苗试验.结果表明MS＋6-BA2mg/L＋0.1NAAmg/L是诱导黄山贡菊芽形成的最佳培养基,茎尖诱导率最高达95％;MS＋6-BA2mg/L＋0.01NAAmg/L是黄山贡菊芽继代增殖的最佳培养基,增殖系数达13.2；在生产上,可采用试管苗瓶外生根方法进行黄山贡菊的快速繁殖.
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| 关 键 词： | 黄山贡菊 组织培养 外植体 |
A Study on Tissue Culture and Rapid Propagation of Dendranthema morifolium cv.＇Gongju＇ |
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| Authors: | Chen Li Wang Zhibing Cao Lingling |
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| Institution: | (School of Life ＆ Environmental Sciences, Huangshan University, Huangshan 245041, China) |
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| Abstract: | Using the shoot-tips, the leaves and the roots of Dendranthema morifolium cv. Gongju as explants, experiments on its bud induction, subculture multiplication and outside-tube rooting and strong seeding were conducted. The results are as follows： the most suitable medium for inducing it was MS＋6- BA2mg/L＋0.1NAAmg/L medium; the highest induction rate of stem tips was 95%; the most suitable medium for subculture multiplication was MS＋6-BA2mg/L＋0.01NAAmg/L, and the modulus was 13.2; the outsidetube rooting technology for test-tube plantlets could be used for rapid propagation of Dendranthema morifolium cv. Gongju. |
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| Keywords: | Dendranthema morifolium cv Gongju tissue cuhure explants |
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