| VNTR技术用于大理地区结核分枝杆菌基因分型的研究 |
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| 引用本文： | 董毅,吴利先.VNTR技术用于大理地区结核分枝杆菌基因分型的研究[J].大理师专学报,2014(8):23-25. |
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| 作者姓名： | 董毅 吴利先 |
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| 作者单位： | 大理学院基础医学院,云南大理671000 |
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| 基金项目： | 国家自然科学基金资助项目（81260456） |
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| 摘 要： | 目的：应用数目可变串联重复序列（VNTR）分子分型技术,对大理地区60株肺结核临床分离株进行分型研究,探讨大理地区菌株DNA多态性及基因型特征。方法：采用VNTR分子分型技术对60株结核分枝杆菌3个VNTR位点进行检测,应用Quantity one软件和BioNumerics 6.6软件进行聚类分析。结果：60株结核分枝杆菌可以分为4个基因群（Ⅰ群、Ⅱ群、Ⅲ群、Ⅳ群）,28个基因型。Ⅰ群占15.0%,含有5个基因型,Ⅱ群占31.7%,含有8个基因型,Ⅲ群占40.0%,含有11个基因型,Ⅳ群占13.3%,含有4个基因型。结论：大理地区的结核分枝杆菌存在基因多态性,其主要流行群为Ⅲ群。
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| 关 键 词： | 结核分枝杆菌 基因分型 数目可变串联重复序列 多态性 大理地区 |
A Study of Genotyping in Mycobacterium tuberculosis Strains in Dali by VNTR Technique |
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| DONG Yi,WU Lixian.A Study of Genotyping in Mycobacterium tuberculosis Strains in Dali by VNTR Technique[J].Journal of Dali Teachers College,2014(8):23-25. |
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| Authors: | DONG Yi WU Lixian |
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| Institution: | (Pre-clinical College, Dali University, Dali,Yunnan 671000,China) |
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| Abstract: | Objective： To explore DNA polymorphism and genotyping features of strains in Dali by investigating the genotyping in Mycobacterium tuberculosis clinical isolates in Dali with variable number tandem repeats（VNTR） analysis. Methods： 3 VNTR loci of 60 Mycobacterium tuberculosis strains were tested with VNTR technology and cluster analysis was performed by Quantity one and BioNumerics 6.6. Results： 60 strains were categorized into 4 gene clusters（I, Ⅱ, Ⅲ and Ⅳ） and 28 genotypes. Cluster Ⅰ accounted for 15.0% including 5 genotypes; Cluster Ⅱ, 31.7% including 8 genotypes; Cluster Ⅲ, 40.0% including 11 genotypes; Cluster Ⅳ, 13.3% including 4 genotypes. Conclusion： There was obvious DNA polymorphism of Mycobacterium tuberculosis clinical strains and Cluster Ⅲ was the predominant prevalent strain in Dali. |
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| Keywords: | Mycobacterium tuberculosis genotyping VNTR polymorphism Dali |
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