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| 川芎嗪对人乳腺癌MCF-7/ADM细胞多药耐药性的逆转及P-糖蛋白表达的影响 | |
| 引用本文： | 解霞,杨毅,高清波,李坤,吕瑜,金爽,彭娜,谢辉.川芎嗪对人乳腺癌MCF-7/ADM细胞多药耐药性的逆转及P-糖蛋白表达的影响[J].大连大学学报,2006,27(4):76-78. |
| 作者姓名： | 解霞 杨毅 高清波 李坤 吕瑜 金爽 彭娜 谢辉 |
| 作者单位： | 1. 大连大学,医学院,辽宁,大连,116022 2. 大连大学,环化学院,辽宁,大连,116022 3. 大连大学,临床医学,辽宁,大连,116022 |
| 摘 要： | 目的]探讨川芎嗪(TMP)对人乳腺癌MCF-7/ADM细胞的耐药逆转及其对该细胞P-糖蛋白(P-gp)表达的影响.方法]MTT法测定细胞的药敏性和抗药性逆转,流式细胞术检测耐药细胞P-糖蛋白的表达.结果]非细胞毒性剂量(320μg/m l)和低毒剂量(1 250μg/m l)的川芎嗪均能显著降低MCF-7/ADM细胞的IC50(P<0.01),逆转倍数分别为2.14和2.80倍;并使该细胞P-gp的表达率由(90.60±0.40)%分别降低至(69.10±1.65)%和(60.30±1.25)%.结论]川芎嗪可部分逆转人乳腺癌MCF-7/ADM细胞对阿霉素的耐药性,其逆转机制与抑制该细胞P-gp的表达有关. |
| 关 键 词： | 川芎嗪 逆转 MCF-7/ADM细胞株 P-糖蛋白 |
| 文章编号： | 1008-2395（2006）04-0076-03 |
| 收稿时间： | 2006-06-13 |
| 修稿时间： | 2006年6月13日 |
Effect of TMP on multidrug resistance reversal and P-gp expression in human breast cancer cell line MCF-7/ADM |
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| XIE Xia,YANG Yi,GAO Qing-bo,LI Kun,L Yu,JIN Shuang,PENG Na,XIE Hui.Effect of TMP on multidrug resistance reversal and P-gp expression in human breast cancer cell line MCF-7/ADM[J].Journal of Dalian University,2006,27(4):76-78. | |
| Authors: | XIE Xia YANG Yi GAO Qing-bo LI Kun L Yu JIN Shuang PENG Na XIE Hui |
| Institution: | XIE Xia,YANG Yi,GAO Qing-bo,LI Kun,L(U) Yu,JIN Shuang,PENG Na,XIE Hui |
| Abstract: | Objective]To investigate the effect of TMP on multidrug resistance reversal and P-glycoprotein(P-gp) expression in human breast cancer MCF-7/ ADM cell line.Methods] The sensitivity of MCF-7/ADM cells and the reversal of resistance were assessed by MTT assay.P-gp expression was determined by flow cytometry.Results] The non-cytotoxic dose(320g/ml) and low-cytotoxic dose(320g/ml) of TMP decreased significantly the IC_(50) value of ADM to MCF-7/ADM cells(P<0.01),and reversed the resistance by 2.14 folds,2.80 folds respectively compared with the IC_(50) value in MCF-7/ADM and reduced P-gp expression from(90.60±0.40)% to(69.10±1.65)% and(60.30±1.25)% respectively.Conclusion]: TMP could partially reverse the resistance to ADM in human breast cancer MCF-7/ ADM cell line,which may be related to inhibiting P-gp expression in MCF-7/ADM cells. |
| Keywords: | TMP reverse MCF-7/ADM cell line P-glycoprotein |
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