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柑桔种质资源谷氨酸草酰乙酸转氨酶同工酶研究
引用本文:钟广炎,叶荫民,陈竹生,吴云伦.柑桔种质资源谷氨酸草酰乙酸转氨酶同工酶研究[J].中国科学院研究生院学报,1993,31(2):162-169.
作者姓名:钟广炎  叶荫民  陈竹生  吴云伦
摘    要:本文分析了125份柑桔属、枳属、金柑属和指来檬属种质资源的谷氨酸草酰乙酸转氨酶同工 酶(GOT)。新发现了GOT-1的两个等位基因B和C。宽皮柑桔的GOT同工酶颇为一致(仅两个例 外),表明可能有共同的起源。酸橙的GOT同工酶较复杂,可能有多种起源,其中朱栾和小红橙的起 源可能与宜昌橙或香橙有关。在单胚种柚中发现了6种GOT基因组合。宜昌橙存在着两种GOT同工酶类型。本文讨论了甜橙、酸橙、葡萄柚、香橙、柠檬等种类的可能起源。

关 键 词:柑桔属  枳属  金柑属  指来檬属  GOT同工酶

A Study on Glutamate Oxaloacetate Transaminase Isozymes of Citrus Germplasm Resources
Zhong Guang-yan,Ye Yin-min,Chen Zhu-sheng,Wu Yun-lun.A Study on Glutamate Oxaloacetate Transaminase Isozymes of Citrus Germplasm Resources[J].Journal of the Graduate School of the Chinese Academy of Sciences,1993,31(2):162-169.
Authors:Zhong Guang-yan  Ye Yin-min  Chen Zhu-sheng  Wu Yun-lun
Abstract:The glutamate oxaloacetate transaminase isozymes (GOT) extracted from 125 biotypes of Citrus and its relatives, Fortunella, Poncirus and Microcitrus were analyzed using polyacrylamide gel electrophoresis to investigate the taxonomic relationships among citrus plants. Besides all the isozymes reported before, two new bands were detected and designated  their putative alleles B and C in GOT-l. Among pummelo cultivars wide variations were found. Most of the mandarins were identical,  having  SS for GOI-1  and MM  for GOT-2  except  for Zou-Pi-Gan and Yao-Gan, which both had FS at GOT-1 and might be hy- brids. This finding suggests that all of the mandarins may have originated from a common ancestor. Sour orange biotypes showed a considerable variation in GOT isozymes. Most of the sour oranges in China were assumed to be hybrids between pummelo and mandarin based on GOT isozyme patterns,  but two biotypes, Xiao-Hong-Cheng and Zhu-lan, had FS at GOT-1 and MB at GOT-2, which strongly suggests that they be hybrids of pummelo and Ichang papeda because B allele of GOT-2 occurs only in Ichang papeda and its close relatives Yuzu and Ichang lemon. From this study Yuzu is assumed to be derived from hybridizationof Ichan, papeda and mandarin.
Keywords:Citrus  Poncirus  Fortunella  Microcitrus  GOT isozymes
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