| 铁-邻菲罗啉分光光度法测定微量葡萄糖 |
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| 引用本文： | 闫家伟,黄运瑞,武江旭,张小强.铁-邻菲罗啉分光光度法测定微量葡萄糖[J].南阳师范学院学报,2004,3(9):42-44. |
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| 作者姓名： | 闫家伟 黄运瑞 武江旭 张小强 |
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| 作者单位： | 南阳师范学院,化学系,河南,南阳,473061 |
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| 摘 要： | 在碱性条件下，建立了测定样品中微量葡萄糖的分光光度法，基于葡萄糖的还原性，用糖将Ag^ 还原为Ag，生成的Ag将Fe^3 还原为Fe^2 ，Fe^2 在pH=4．5的介质中与邻菲罗啉(o-phell)反应形成桔红色络合物，在510nm波长处有一最大吸收。
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| 关 键 词： | 葡萄糖 邻菲罗啉 分光光度法 |
| 文章编号： | 1671-6132(2004)09-0042-03 |
| 修稿时间： | 2004年2月20日 |
Spectrophotometric Determination of Mico glucose with Iron-o-phenanthrollne |
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| YAN Jia-wei,HUANG Yun-rui,WU Jiang-xu,ZHANG Xiao-qiang.Spectrophotometric Determination of Mico glucose with Iron-o-phenanthrollne[J].Journal of Nanyang Teachers College,2004,3(9):42-44. |
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| Authors: | YAN Jia-wei HUANG Yun-rui WU Jiang-xu ZHANG Xiao-qiang |
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| Abstract: | Be lining below the alkalinity,a method for the determination of micro glucose by spectrophometry was estblished,based on using glucose as a reduction agent,Ag~+ could be reduced Ag,Ag could reduced Fe~(3+),which reacted with o-phenantroline(o-phen)in the medium of pH4.5 to form an orange complex with a maximum absorption at 510nm.The method has heen applied to determine micro glucose in the samples satisfactory results. |
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| Keywords: | glucose o-phenathroline s-ectrophotomety |
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