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低色素出芽短梗霉W2003发酵条件研究
引用本文:潘素娟,王建军,焦成谨,汪之波,漆爱红,王长青.低色素出芽短梗霉W2003发酵条件研究[J].天水师范学院学报,2010,30(2):59-62.
作者姓名:潘素娟  王建军  焦成谨  汪之波  漆爱红  王长青
作者单位:天水师范学院生命科学与化学学院,甘肃,天水,741001
摘    要:出芽短梗W2003是一株天然的产色素能力弱化菌株.通过单因素实验和正交优化实验确定了W2003菌株的最佳发酵培养条件为:28℃,转速为150r/min,初始pH为6.5,培养时间60h;培养基配方组分为(g/L):蔗糖70,酵母浸膏10,KH2PO42.0,MgSO4·7H2O1.0,NaOH1.0,(NH4)2SO40.04.在优化条件下该菌株的多糖转化率为16.68%,培养液多糖含量为11.68g/L.

关 键 词:出芽短梗霉  低色素  发酵条件

Studies on the Fermentation Conditions of Aureobasidiun pullulans W2003 for Producing Low Pigment
Pan sujuan,Wang jianjun,Jiao chengjin,Wang zhibo,Qi aihong,Wang changqing.Studies on the Fermentation Conditions of Aureobasidiun pullulans W2003 for Producing Low Pigment[J].Journal of Tianshui Normal University,2010,30(2):59-62.
Authors:Pan sujuan  Wang jianjun  Jiao chengjin  Wang zhibo  Qi aihong  Wang changqing
Institution:(School of Life Science and Chemistry, Tianshui Normal University, Tianshui Gansu 741001, China)
Abstract:Aureobasidium pullulans W2003 is a natural strain of producing pigment-free. The best fermentation conditions were determined by the method of single factor tests and orthogonal optimization experiments in this paper. The optimized culture conditions were as follows: 28℃, 150r/min, pH= 6.5, culture time 60h; Medium composition (g / L): sucrose 70, yeast extract 10, KH2PO4 2.0, MgSO4·7H2O 1.0, NaOH 1.0, (NH4)2SO4 0.04. Under these conditions, polysaccharide content was 11.68g in culture fluid and the polysaccharide conversion rate was 16.68%.
Keywords:Aureobasidiun pullulans W2003  pigment-free  fermentation condition
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