| Scale-up of rifamycin B fermentation with Amycolatoposis mediterranei |
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| 作者姓名： | 金志华 林建平 CEN Pei-lin 岑沛霖 |
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| 作者单位： | Institute of Bioengineering，School of Materials Science and Chemical Engineering，Zhejiang University，Hangzhou 310027，China，(Department of Bioengineering and Pharmaceutical Engineering，Ningbo Institute of Technology，Zhejiang University，Ningbo 315100，China，Institute of Bioengineering，School of Materials Science and Chemical Engineering，Zhejiang University，Hangzhou 310027，China，Institute of Bioengineering，School of Materials Science and Chemical Engineering，Zhejiang University，Hangzhou 310027，China，Institute of Bioengineering，School of Materials Science and Chemical Engineering，Zhejiang University，Hangzhou 310027，China |
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| 摘 要： | Study of the effect of dissolved oxygen and shear stress on rifamycin B fermentation with A. mediterranei XC 9-25 showed that rifamycin B fermentation with Amycolatoposis mediterranei XC 9-25 needs high dissolved oxygen and is not very sensitive to shearing stress. The scale-up of rifamycin B fermentation with A mediterranei XC 9-25 from a shaking flask to a 15 L fermentor was realized by controlling the dissolved oxygen to above 25% of saturation in the fermentation process, and the potency of rifamycin B fermentation in the 15 L fermentor reached 10 g/L after 6-day batch fermentation. By continuously feeding glucose and ammonia in the fermentation process, the potency of rifamycin B fermentaion in the 15 L fermentor reached 18.67 g/L, which was 86.65% higher than that of batch fermentation. Based on the scale-up principle of constantly aerated agitation power per unit volume, the scale-up of rifamycin B fed-batch fermentation with continuous feed from a 15 L fermentor to a 7 m3 fermentor and further to
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Scale-up of rifamycin B fermentation with Amycolatoposis mediterranei |
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| JIN Zhi-hua LIN Jian-ping,CEN Pei-lin.Scale-up of rifamycin B fermentation with Amycolatoposis mediterranei[J].Journal of Zhejiang University Science,2004(12). |
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| Authors: | JIN Zhi-hua LIN Jian-ping CEN Pei-lin |
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| Abstract: | Study of the effect of dissolved oxygen and shear stress on rifamycin B fermentation with A. mediterranei XC 9-25 showed that rifamycin B fermentation with Amycolatoposis mediterranei XC 9-25 needs high dissolved oxygen and is not very sensitive to shearing stress. The scale-up of rifamycin B fermentation with A mediterranei XC 9-25 from a shaking flask to a 15 L fermentor was realized by controlling the dissolved oxygen to above 25% of saturation in the fermentation process, and the potency of rifamycin B fermentation in the 15 L fermentor reached 10 g/L after 6-day batch fermentation. By continuously feeding glucose and ammonia in the fermentation process, the potency of rifamycin B fermentaion in the 15 L fermentor reached 18.67 g/L, which was 86.65% higher than that of batch fermentation. Based on the scale-up principle of constantly aerated agitation power per unit volume, the scale-up of rifamycin B fed-batch fermentation with continuous feed from a 15 L fermentor to a 7 m3 fermentor and further to a 60 m3 fermentor was realized successfully. The potency of rifamycin B fermentation in the 7 m3 fermentor and in the 60 m3 fermentor reached 17.25 g/L and 19.11 g/L, respectively. |
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| Keywords: | Amycolatoposis mediterranei Fermentation Rifamycin B Scale-up |
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