| 春小麦外源DNA两次导入和导入后杂交及其后代醇溶蛋白电泳分析 |
| |
| 引用本文： | 梁顺祥,许永财,迟德钊,黄居茂.春小麦外源DNA两次导入和导入后杂交及其后代醇溶蛋白电泳分析[J].青海科技,1998(2). |
| |
| 作者姓名： | 梁顺祥 许永财 迟德钊 黄居茂 |
| |
| 作者单位： | 青海大学农学系!西宁，810016，青海大学农学系!西宁，810016，青海省农林科学院作物所!西宁，810016，青海省农林科学院作物所!西宁，810016 |
| |
| 摘 要： | 本文采用A－PAGE方法，对外源DNA导入两次的四个后代变异系、一个后代变异株系的六个单株和导入后代间杂交的四个后代变异系进行了醇溶蛋白电泳分析。结果表明：1．外源DNA两次导入进一步强化了变异；2．A－PAGE方法不仅可用于验证外源DNA导入的变异后代，而且可检测后代变异系的遗传稳定性；3．使外源DNA导入后的变异后代间再进行杂交，能够丰富变异类型。而且导入技术与杂交方法的结合，可以互相补充，对作物育种具有重要意义。
|
| 关 键 词： | 春小麦 外源DNA导入 杂交 醇溶蛋白 电泳 |
GLIADIN ANALYSIS WITH THE A-PAGE METHOD ON THE VARIANT PROGENY OF SPRING WHEAT INTRODUCED EXOGENOUS DNA AGAIN AND HYBRIDIZED BETWEEN TWO INTRODUCED DESCENDANTS |
| |
| Liang Shunxiang, Xu Yongcai.GLIADIN ANALYSIS WITH THE A-PAGE METHOD ON THE VARIANT PROGENY OF SPRING WHEAT INTRODUCED EXOGENOUS DNA AGAIN AND HYBRIDIZED BETWEEN TWO INTRODUCED DESCENDANTS[J].Qinghai Science and Technology,1998(2). |
| |
| Authors: | Liang Shunxiang Xu Yongcai |
| |
| Institution: | Liang Shunxiang; Xu Yongcai (Agriculture Department of Qinghai University Xining 810016)Chi Dezhao Huang Jumao(Qinghai Academy of Agriculture and Forestry Xining 810016) |
| |
| Abstract: | The spring wheat seed gliadin of variant systems introduced exogenous DNA again and that hybridized between two introduced descendants has been analysed with the A-PAGE method. The results shows: 1. Introducing exogenous DNA again has intensified the variation. 2. The A-PAGE method can be applied not only to verify variant progeny introduced exogenous DNA,but to examine genetic stability of variant progeny. 3. The combination of introduction and hybridization technique has important significance on crop breeding. |
| |
| Keywords: | Spring Wheat Exogenous DNA Introduction Hybridization Gliadin Electrophoresis |
| 本文献已被 CNKI 等数据库收录！ |
