| 液体培养法提高太子参组培苗质量的研究 |
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| 引用本文： | 周雪林,徐龙飞,周庆萍,张新.液体培养法提高太子参组培苗质量的研究[J].六盘水师范高等专科学校学报,2013(4):24-26. |
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| 作者姓名： | 周雪林 徐龙飞 周庆萍 张新 |
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| 作者单位： | 六盘水师范学院 生命科学系,贵州 六盘水,553001 |
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| 基金项目： | 六盘水师范学院重点学科建设(No.:52020-2012-05-01-02)。六盘水师范学院重点学科建设(No.:52020-2012-05-01-02)。 |
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| 摘 要： | 受无糖培养技术启示，在太子参组培苗育苗过程中，以液体培养基取代固体培养基，应用纸桥法，以太子参幼苗茎段为外植体，经有效诱导芽分化后转入以蛭石为基质的增殖培养基增殖培养，并诱导生根，成苗后辅以自然光照，通过培养基改进对太子参组培苗质量进行研究。结果表明：茎段在合MS＋0．1mg／LNAA＋0．5～1．0mg／L6-BA＋2％蔗糖的液体培养基滤纸桥上的芽分化率达80％以上。不定芽在以蛭石为基质的MS＋0．1mg／LNAA＋1mg／L6．BA十2％蔗糖的培养基中增殖并生根，生根后的试管苗转到低糖（0．5％）或无糖培养基中，经自然光照培养能得到高质量的试管苗。
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| 关 键 词： | 太子参 液体培养 组培苗 质量 |
Liquid Culture Method Improve Quality of Tissue-cultured Seedling of Pseudostellaria Heterophylla |
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| ZHOU Xue-lin
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XU Long-fei
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ZHOU Qing-ping
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ZHANG Xin.Liquid Culture Method Improve Quality of Tissue-cultured Seedling of Pseudostellaria Heterophylla[J].Journal of Liupanshui Teachers College,2013(4):24-26. |
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| Authors: | ZHOU Xue-lin XU Long-fei ZHOU Qing-ping ZHANG Xin |
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| Institution: | ZHOU Xue-lin; XU Long-fei; ZHOU Qing-ping ; ZHANG Xin (Department of Life Sciences, Liupanshui Normal University, Liupanshui 553001, China) |
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| Abstract: | Inspiraed by sugar-free culture technique, liquid culture medium replacement for solid culture medi- um, the stems of pseudostellaria heterophylla have been used as explants which incubate on filter paper bridg- es in test tubes containing liquid basal medium to study quality in tissue-cultured shoots. The results show that MS＋0. lmg/L NAA＋0.5 ~ 1.0mg/L 6-BA＋2% sucrose was liquid basal medium for filter paper bridges, and the shoot differentiation rate was up to 80%. The adventitous sprouts are propagated and rooted in ver- miculite medium of MS＋0. 1 mg/L NAA＋ 1mg/L 6-BA＋2% sucrose. Then high quality test-tube plantlets were cultivated in low sugar （0.5%） or sugar-free culture medium by natural lighting. |
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| Keywords: | pseudostellaria heterophylla liquid culture tissue culture seedling quality |
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