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Blast transformation of lymphocytes stimulated with phytohaemagglutinin in dilated cardiomyopathy (DCM) patients |
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| Authors: | R L Tripathi K K Talwar K S S Saibaba L M Srivastava |
| Institution: | 1. In Service Training Programme MLT Unit & Hospital Laboratory Services, Deptt. of Biochemistry UCMS & G.T.B. Hospital, Shahdara, 110095, Delhi 2. Department of Cardiology, All India Institute of Medical Sciences, 110 029, New Delhi 3. Department of Biochemistry, Sri Venkateswara Institute of Medical Sciences, 517 507, Tirupati, Andhra Pradesh 4. Department of Biochemistry, All India Institute of Medical Sciences, 110 029, New Delhi |
| Abstract: | Blast transformation of peripheral blood lymphocytes (PBL) in dilated cardiomyopathy (DCM) patients and normal controls was examined to assess the cell mediated immune response of these patients. Uptake of3H-thymidine by PBL of DCM patients incubated for 72 hrs with phytohaemagglutinin (PHA) was 17787±9513 CPM and without PHA was 1475±529 CPM showing a stimulation index (SI) by PHA of 12.95±6.35. The corresponding values in healthy controls were 55078±12282 and 1868±792 respectively with a SI of 38.0±26.0. Blast transformation of PBL by PHA was significantly suppressed in DCM patients as compared to normal controls. This suppression was found to be highly significant and possibly indicates the defective cell medicated immune response in DCM patients. |
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