|
|||||
|
|
| 复方中药对大鼠膝关节软骨缺损修复作用的实验研究 | |
| 引用本文： | 胡毓诗,张挥武,王传恩,冉德州.复方中药对大鼠膝关节软骨缺损修复作用的实验研究[J].成都体育学院学报,2005,31(5):100-103. |
| 作者姓名： | 胡毓诗 张挥武 王传恩 冉德州 |
| 作者单位： | 1. 成都体育学院运动医学系,四川,成都,610041 2. 四川省骨科医院,四川,成都,610041 3. 成都体育学院附属医院,四川,成都,610041 |
| 摘 要： | 研究目的:通过口服补肝肾、续筋骨类中药,观察该药对大鼠膝关节股骨髁间凹软骨全层缺损的修复情况,探讨该类中药对软骨缺损修复的影响.研究方法:选用sD大鼠42只,以手术钻孔的方法,造成左膝股骨髁间全层软骨缺损.实验组每日两次灌服复方中药3ml,对照组无治疗,于术后3周、6周、9周,各处死大鼠7只,取股骨下端,作H.E染色、美蓝染色,观察组织学改变,并作胶原Ⅱ型和肝细胞生长因子(hepatocyte growth factor ,HGF)的免疫组化染色.研究结果:组织学结果显示,9周时实验组组织学修复优于对照组;实验组Ⅱ型胶原表达较对照组有增强的趋势;HGF在软骨细胞胞浆表达,且发现肌肉组织的肌浆中有阳性表达.研究结论:运用中医补肝肾、强筋骨的方法,可以改善局部营养供应,提高代谢机能,从而达到大鼠膝关节软骨缺损修复的目的. |
| 关 键 词： | 膝关节软骨缺损 补肝肾中药 肝细胞生长因子(HGF) 胶原Ⅱ型 |
| 文章编号： | 1001-9154（2005）05-0100-04 |
| 收稿时间： | 2005-06-16 |
| 修稿时间： | 2005年6月16日 |
Experimental Research on the Effect of Chinese Herb in Repairing Defect of Articular Cartilage of the Knee |
|
| HU Yu-shi, et al.Experimental Research on the Effect of Chinese Herb in Repairing Defect of Articular Cartilage of the Knee[J].Journal of Chengdu Physical Education Institute,2005,31(5):100-103. | |
| Authors: | HU Yu-shi |
| Institution: | Sports Medical Department, Chengdu Sport University, Chengdu 610041 |
| Abstract: | Objective To observe the effect of Chinese herbs in repairing the defect of articular cartilage of the knee,and try to discuss its mechanism.Methods The drill,full-thick articular cartilage defects were produced in the left knee joints of 42 rats.Every rat of experimental group was feeding with Chinese herbs 3ml two times a day as treated.7 animals were killed respectively in 3,6 and 9 weeks,and the histological observation,H.E pigmentation and immunohistochemical examination were performed.Immunohistochemical staining of type-II collagen and hepatocyte growth factor(HGF) were analyzed by image-pro-plus system.Result The repairing tissue in experimental group was better than control group.The Expression of type-II collagen increased than control group.HGF expressed in cartilage cell plasm,and showed positive in muscle plasm.Conclusion Chinese herbs is nourishing and enriching the liver and kidney,it plays an important role in repairing defect of articular cartilage of the knee.The effective mechanism is to replenish materials,and strengthen the physiological function. |
| Keywords: | defect of articular cartilage of knee joint Chinese herbs of enriching liver and kidney hepatocyte growth factor(HGF) type-II collagen |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |