| DPPH和FRAP法测定41种中草药抗氧化活性 |
| |
| 引用本文： | 陈玉霞,刘建华,林峰,杜向党.DPPH和FRAP法测定41种中草药抗氧化活性[J].实验室研究与探索,2011,30(6). |
| |
| 作者姓名： | 陈玉霞 刘建华 林峰 杜向党 |
| |
| 作者单位： | 河南农业大学牧医工程学院,河南郑州,450002 |
| |
| 基金项目： | “十一五”国家科技支撑计划资助项目(2006BAK02A03-3); 河南省青年骨干教师资助计划 |
| |
| 摘 要： | 为了测定41种抗衰老中草药的抗氧化活性,筛选了抗氧化作用强的中草药。实验采用70%的乙醇作溶剂,超声细胞破碎仪对药物进行提取,抗氧化能力的测定使用分光光度计,应用DPPH和FRAP进行测定。通过测定41种中草药乙醇提取液对DPPH自由基的清除能力和FRAP值,表征其抗氧化能力。结果表明:何首乌、大黄、淫羊藿、山楂、白芍、山茱萸等在10 mg/mL的浓度下,对DPPH自由基的清除率超过70%,总抗氧化能力的FRAP值>200,抗氧化能力较强;其他药物也有不同程度的抗氧化活性,但作用较弱。
|
| 关 键 词： | 分光光度法 中草药 FRAP法 DPPH法 抗氧化活性 |
Determination of Antioxidative Activity of 41 Kinds of Chinese Herbal Medicines by Using DPPH and FRAP Methods |
| |
| CHEN Yu-xia,LIU Jian-hua,LIN Feng,DU Xiang-dang.Determination of Antioxidative Activity of 41 Kinds of Chinese Herbal Medicines by Using DPPH and FRAP Methods[J].Laboratory Research and Exploration,2011,30(6). |
| |
| Authors: | CHEN Yu-xia LIU Jian-hua LIN Feng DU Xiang-dang |
| |
| Institution: | CHEN Yu-xia,LIU Jian-hua,LIN Feng,DU Xiang-dang(College of Animal Husbandry and Veterinary,Henan Agricultural University,Zhengzhou 450002,China) |
| |
| Abstract: | The antioxidative activities of 41 kinds of Chinese herbal medicines were determined and selected.The antioxidative substances were extracted from Chinese herbal medicines by 70 percent ethanol and omni ruptor.The spectrophotometric method was used to study the antioxidant activities of medicinal herbs by DPPH and FRAP methods.The results show that these kinds of medicinal herbs including Polygonum multiflorum,Rheum pamatum,Epimedium grandiforum,Crataegus tifiga,Paeonia lactiflora,Cornus officinalis have st... |
| |
| Keywords: | spectrophotometry Chinese herbal medicines DPPH method FRAP method antioxidative activity |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
