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力竭游泳运动对肠组织MDA、Free-SH、ATP含量、Na+-K+-ATPase和Ca2+-ATPase活性的影响
引用本文:李风华,王宝明,衣雪洁.力竭游泳运动对肠组织MDA、Free-SH、ATP含量、Na+-K+-ATPase和Ca2+-ATPase活性的影响[J].沈阳体育学院学报,2011,30(3):72-76.
作者姓名:李风华  王宝明  衣雪洁
作者单位:1. 北京工业职业技术学院,北京,100042
2. 沈阳体育学院 运动人体科学学院,辽宁 沈阳,110102
摘    要:探讨力竭性运动引起的氧化应激对肠功能的影响及运动性肠功能紊乱的原因,32只SD雄性大鼠随机分为4组:即对照组(C);运动后即刻组EX;运动后30min组(EX30);运动后60min组(EX60),测定力竭性游泳后不同时相肠组织匀浆MDA、游离巯基和ATP含量。结果:运动后肠组织MDA含量在运动后30min、60min显著性增加(P<0.01);运动后Free-SH含量在运动后30min(P<0.05)和运动后60min后(P<0.01)显著下降;运动后30min组ATP含量显著下降(P<0.01);运动后30min,Ca2+-ATPase活性下降(P<0.05);运动后60min,Na+-K+-AT-Pase和Ca2+-ATPase活性均显著下降(P<0.05)。结论:运动源性自由基产生增加,使肠组织中游离巯基被氧化,导致ATP含量下降,Na+-K+-ATPase和Ca2+-ATPase活性下降,可能是造成运动性肠功能紊乱的重要因素之一。

关 键 词:力竭性游泳  丙二醛  游离巯基  ATP  肠组织  Na+-K+-泵  Ca2+-泵

Effects of Exhaustive Swimming on Lipid Peroxidation,Free Thiol Group,ATP Content,Na~+-K~+-ATPase and Ca~(2+)-ATPase Activity in the Rat's Intestine Tissue
LI Fenghua,WANG Baoming,YI Xuejie.Effects of Exhaustive Swimming on Lipid Peroxidation,Free Thiol Group,ATP Content,Na~+-K~+-ATPase and Ca~(2+)-ATPase Activity in the Rat's Intestine Tissue[J].Journal of Shenyang Sport University,2011,30(3):72-76.
Authors:LI Fenghua  WANG Baoming  YI Xuejie
Institution:LI Fenghua1,WANG Baoming2,YI Xuejie2(1.Beijing Polytechnic College,Beijing 100042,China,2.School of Human Exercise Science,Shenyang Sport University,Shenyang 110102,Liaoning,China)
Abstract:In order to investigate the effects of oxidative stress on intestines function and the exercise-induced intestines function disorder,32 male SD rats were divided into four groups(control group,immediate postexercise group,30 min postexercise group and 60 min postexercise group).We measured contents of MDA,FREE-SH and ATP contents in rats intestine tissues.The result showed that MDA contents had a significant increase at 30 min and 60 min after exhaustive swimming while FREE-SH contents had a remarkable decr...
Keywords:exhaustive swimming  MDA  FREE-SH  ATP  intestine tissue  Na -K -ATPase  Ca2 -ATPase  
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