| 应用地高辛标记的cRNA探针原位杂交法检测大鼠下丘脑生长抑素mRNA |
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| 引用本文： | 李英,周济远,任君旭,王建伟,白雪梅,张耕.应用地高辛标记的cRNA探针原位杂交法检测大鼠下丘脑生长抑素mRNA[J].河北北方学院学报(医学版),1996(1). |
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| 作者姓名： | 李英 周济远 任君旭 王建伟 白雪梅 张耕 |
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| 作者单位： | 张家口医学院组胚教研室
(李英，周济远，任君旭，王建伟，白雪梅)，张家口医学院组胚教研室(张耕) |
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| 摘 要： | 用成年雄性Wistar大鼠,经4％多聚甲醛灌注固定,取下丘脑组织,行再固定24h,常规石蜡包埋和切片,用地高辛标记的cRNA探针进行厚位杂交,结果显示在弓状核及其邻近区域见到阳性神经元,阳性信号为蓝色颗粒,胞质、核膜及核仁呈阳性 阴性对照均无阳性反应出现 本实验证实,用石蜡切片进行原位杂交组织化学研究,能检测下丘脑生长抑素神经元基因表达产物mRNA,方法稳定,重复性好,便于普通实验室开展工作。
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| 关 键 词： | 生长抑素mRNA 地高辛标记的cRNA 探针 下丘脑 原位杂交 |
DETECTION OF SOMATOSTATIN mRNA BY IN SITU HYBRIDIZATION USING A DIGOXIGEN LABELED cRNA PROBE |
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| Li Ying et al.DETECTION OF SOMATOSTATIN mRNA BY IN SITU HYBRIDIZATION USING A DIGOXIGEN LABELED cRNA PROBE[J].Journal of Hebei North University:Medical Edition,1996(1). |
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| Authors: | Li Ying |
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| Institution: | Li Ying et al |
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| Abstract: | The rats were perfused with 4% paraformalclehyde. The medialbasalhypothalami were
dissected and fixed for 24h m the same fixative. Thespeoimens were ombedded m paraffin and sectioned convenlionlly Theresults revealcd thal in seclions hvbncdzed with somatostalin cRNAprobe, the positive neuron were seen in arcuate nucleus and itsadjacent area. the positive signals were blue m color and located incvloplasm memherone of nucleus and niirlcolus of neurons. The controlseelions showed negative reactlon. The results indicated thal themethod with in situ hyhridization to paraffin sectton is practical andeasy to re-peat, the results are riliable to detecl somatostatm mRNA. |
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| Keywords: | somatostation mRNA digoxigcnm - labeled cRNA probehypothalaamus in situ hybndization |
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