| 强脉冲电场致细胞膜电穿孔的实验研究 |
| |
| 引用本文： | 肖华娟,严萍,牟群.强脉冲电场致细胞膜电穿孔的实验研究[J].中国科学院研究生院学报,2005,22(4):462-466. |
| |
| 作者姓名： | 肖华娟 严萍 牟群 |
| |
| 作者单位： | 1. 中国科学院电工研究所,北京,100080;中国科学院研究生院,北京,100049
2. 中国科学院电工研究所,北京,100080 |
| |
| 基金项目： | 国家自然科学基金项目 ( 5 0 44 70 0 7)资助 |
| |
| 摘 要： | 采用肝癌细胞HePG2作为靶细胞,罗丹明偶联葡聚糖为荧光探针,荧光分光光度计作为检测设备,试验研究细胞膜电穿孔与电脉冲参数之间的关系.试验中发现随着电场强度或脉冲宽度的增加,样品荧光强度增加,细胞膜穿孔率相应增强;同时发现细胞膜电穿孔效应具有累积效应
|
| 关 键 词： | 细胞膜电穿孔 强脉冲电场 生物效应 |
| 文章编号： | 1002-1175(2005)04-0462-05 |
| 修稿时间： | 2004年3月18日 |
The Experimental Study of Electroporation of Cell Membrane Induced by Pulsed Electric Field of High Intensity |
| |
| XIAO Hua-Juan,YAN Ping,MU Qun.The Experimental Study of Electroporation of Cell Membrane Induced by Pulsed Electric Field of High Intensity[J].Journal of the Graduate School of the Chinese Academy of Sciences,2005,22(4):462-466. |
| |
| Authors: | XIAO Hua-Juan YAN Ping MU Qun |
| |
| Institution: | XIAO Hua-Juan 1,2 YAN Ping 1 MU Qun 1 |
| |
| Abstract: | In this paper, cell membrane electroporation induced by pulsed electric field is studied by using liver cancer cells as target cells, Rhodamine B isothiocyanate-Dextran as fluorescent probes and fluorescence spectrophotometer as measure equipment. The experiments have shown that when the pulse intensity or pulse width increases, the fluorescent intensity will increase accordingly, so will the rate of electroporation of cell membranes. Also we find that electroporation of cell membrane presents memory effects. |
| |
| Keywords: | electroporation of cell membrane high intense pulsed electric field biological effect |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
