| L-苯丙氨酸对铜绿微囊藻生长的作用与机制 |
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| 引用本文： | 韦海燕,杨飞,尹立红,浦跃朴.L-苯丙氨酸对铜绿微囊藻生长的作用与机制[J].东南大学学报,2011(4):445-448. |
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| 作者姓名： | 韦海燕 杨飞 尹立红 浦跃朴 |
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| 作者单位： | 东南大学环境医学工程教育部重点实验室 |
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| 基金项目： | The National Science and Technology Major Project(No.2009ZX07101-011);the National Natural Science Foundation of China(No.30972440) |
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| 摘 要： | 应用细胞计数法结合流式细胞术探讨了L-苯丙氨酸对铜绿微囊藻生长的作用与可能机制.结果表明:作用24 h后,较低浓度L-苯丙氨酸(0.078 ～0.312 μg/mL)对藻细胞生长表现为促进作用,而在较高浓度(0.625 ～ 20μg/mL)时表现为抑制作用.L-苯丙氨酸对铜绿微囊藻生长的抑制作用存在剂量效应和时间效应关...
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| 关 键 词： | 控藻 L-苯丙氨酸 机制 流式细胞术 膜 生长 |
Effects and mechanisms of L-phenylalanine on growth of Microcystis aeruginosa |
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| Wei Haiyan Yang Fei Yin Lihong Pu Yuepu.Effects and mechanisms of L-phenylalanine on growth of Microcystis aeruginosa[J].Journal of Southeast University(English Edition),2011(4):445-448. |
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| Authors: | Wei Haiyan Yang Fei Yin Lihong Pu Yuepu |
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| Institution: | Wei Haiyan Yang Fei Yin Lihong Pu Yuepu(Key Laboratory of Environmental Medicine Engineering of Ministry of Education,Southeast University,Nanjing 210009,China) |
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| Abstract: | The effects and possible mechanisms of action of L-phenylalanine on the growth of Microcystis aeruginosa cells were explored by cell counting and flow cytometry assays.L-phenylalanine promoted the growth of Microcystis aeruginosa at concentrations between 0.078 and 0.312 μg/mL,but inhibited growth at concentrations between 0.625 and 20 μg/mL in 24 h exposure.The dose-effect and time-course relationships between exposure to L-phenylalanine and growth inhibition of Microcystis aeruginosa were observed.The IC50 value of L-phenylalanine for growth inhibition of Microcystis aeruginosa was 6.2 μg/mL(95% confidence interval was 0.005 to 16.76 μg/mL).The membrane integrity of the cells showed significant variations after 24 h exposure to L-phenylalanine.Meanwhile,no effects on esterase activity of the cells were observed until after 48 h exposure to L-phenylalanine.In conclusion,L-phenylalanine has hormesis effects and algae control effects on Microcystis aeruginosa.The latter is closely related to alterations or disorders in the cell membrane and with variation of esterase activity in the cells. |
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| Keywords: | algae control L-phenylalanine mechanisms flow cytometry membranes growth |
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