| 新型铱配合物组氨酸荧光传感器性质探析 |
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| 引用本文： | 肖光参.新型铱配合物组氨酸荧光传感器性质探析[J].闽江学院学报,2014,35(5):107-112. |
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| 作者姓名： | 肖光参 |
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| 作者单位： | 福州大学测试中心,福建福州,350002 |
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| 基金项目： | 福建省高校产学合作科技重大项目 |
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| 摘 要： | 根据铱配合物1-CF3SO3与组氨酸可以特异性结合并产生强荧光的特性,建立了一种定量检测组氨酸的新方法.缓冲体系类型对1-CF3SO3和组氨酸共同产生的荧光影响不大,体系荧光在混合反应50 min后趋于稳定.体系的荧光强度与组氨酸浓度在1.0 ～13.0 μmol范围内呈良好的线性关系,检测限为0.3 μmol.同时通过加标回收的方法研究了该方法的准确性,为组氨酸的定量分析提供了一种新的方法.
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| 关 键 词： | 新型铱配合物 组氨酸 荧光传感器 |
Analysis of a novel iridium complexes for histidine fluorescent sensor |
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| XIAO Guang-can.Analysis of a novel iridium complexes for histidine fluorescent sensor[J].Journal of Minjiang University,2014,35(5):107-112. |
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| Authors: | XIAO Guang-can |
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| Institution: | XIAO Guang-can ( Testing Center,Fuzhou University, Fuzhou, Fujian 350002, China) |
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| Abstract: | In this paper, based on the characters that the interaction of iridium complex and histidine can emit a strong fluorescence, a quantitavie analysis method for histidine had been developed. The experimental results showed that the strongest fluorescence intensity could be reached under the follow- ing condition： iridium complex/hisditine = 1/4, neutral reaction medium and 50 min reaction time. Under the optimized conditions, the fluorescence intensities of the system had a linear relationship with the histidine concentration in the range of 1.0 ～13.0 μmol with the detection limit of 0.3 μmol. The accuracy of the method was also studied by spiking recovery method for quantitative histidine provides a new method to analyze. |
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| Keywords: | iridium complex histidine fluorescent sensor |
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