| 基于Nafion固定天青I和纳米CdS共修饰玻碳电极的H2O2生物传感器 |
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| 引用本文： | 李华刚.基于Nafion固定天青I和纳米CdS共修饰玻碳电极的H2O2生物传感器[J].黔南民族师范学院学报,2008,28(3):68-72. |
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| 作者姓名： | 李华刚 |
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| 作者单位： | 遵义师范学院化学系,贵州遵义563002 |
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| 摘 要： | 利用滴涂于玻碳电极（GCE）表面的以Nnfion膜中负电性的磺酸基与天青I（AI）阳离子之间的静电作用，实现天青I在电极上的固定化，再通过静电吸附和自组装技术将纳米CdS吸附的辣根过氧化物酶（HRP）修饰到电极表面，制备出性能良好的H2O2生物传感器。采用循环伏安法（CV）和计时电流法考察了该传感器的电化学性质。实验表明，该传感器具有良好的生物催化活性，较高的灵敏度，良好的选择性和稳定性，且易于制作等特点。响应电流与H2O2浓度在8．0×10^-6～1．6×10^-3 moL／L范围呈现良好的线性关系，检出限为2．72×10^-6 moL／L。
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| 关 键 词： | Nation 天青I 纳米CdS 辣根过氧化物酶 生物传感器 |
H202 Biosensor Based on Azure I Incorporated into Nation Film and CdS Modified Glassy Carbon Electrode |
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| Institution: | LI Hua -gang ( Dept. of Chemistry, Zunyi Normal College, Zunyi 563002, China) |
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| Abstract: | The H2O2 biosensor was prepared from a Nation film coated Glassy Carbon Electrode（GCE）. The Azure I was immobilized via electrostatic force between the negatively charged sulfonic acid groups in Nation film and the cation of Azure I. The horseradish peroxidase （HRP） adsorption on CdS nanoparticles was modified to the surface of glassy carbon electrode to form a good H2 O2 Biosensor by self- Assembly technology and electrostatic. The electrochemical characteristics of the biosensor were characterized by cyclic vohammetry （CV） and chronoarnpermetry. The experimental results indicated that the sensor possessed high catalytic activity, relatively higher sensitivity, better selectivity, stability, and easy production. The response current was linear with the concentration of H2O2 antigen in the range of 8.0× 10^-6 and h 6×10^13 moL/L respectively under the optimum conditions, and the detection limit was found to be 2.72×10^-6moL/L. |
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| Keywords: | Nation Azure I CdS nanoparticles horseradish peroxidase biosensor |
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