| 营养盐对小球藻生长及胞内蛋白质含量的影响 |
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| 引用本文： | 于媛,刘艳,杨海波,张欣华,李英敏,吕福荣,林芃.营养盐对小球藻生长及胞内蛋白质含量的影响[J].大连大学学报,2002,23(6):12-16. |
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| 作者姓名： | 于媛 刘艳 杨海波 张欣华 李英敏 吕福荣 林芃 |
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| 作者单位： | 1. 大连大学化学与化学与工程系,辽宁,大连,116622
2. 大连大学生物工程学院,辽宁,大连,116622 |
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| 基金项目： | 辽宁省博士科研启动基金资助项目(001059) |
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| 摘 要： | 微藻含有的蛋白质、糖蛋白等活性物质具有许多重要的生物活性和生理功能.在24～26℃、自然光照的条件下,首先考察小球藻生长与胞内蛋白质积累的关系,其次利用正交设计实验考察不同浓度的营养盐对小球藻生长及胞内蛋白质含量的影响.结果表明,小球藻中蛋白质的合成与积累主要发生在生长过程的平衡期.利于小球藻生长的营养盐的优化组合为:C6H12O6:2g/L,KNO3:0.8g/L,Na2PO4:0.2g/L,细胞的最大密度为6.46×107个细胞/L培养液.获得最高蛋白质含量的营养盐的优化组合为:C6H12O6:6g/L,KNO3:1.6g/L,NaH2PO4:0.2g/L,与之对应蛋白质含量为:655.4mg/g.此结果为进一步从微藻中获取单一组成的,具有重要生物和生理活性的蛋白质及糖蛋白提供了优化培养微藻的实验依据和规模化生产的基础条件.
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| 关 键 词： | 小球藻 营养盐 蛋白质 正交设计 |
| 文章编号： | 1008-2395(2002)06-0012-05 |
| 修稿时间： | 2002年10月17 |
The effects of nutritions on the C. vulgaris growth and the content of protein |
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| YU Yuan,LIU Yan,YANG Hai-bo,ZHANG Xin-hua,LI Ying-min,LU Fu-Rong,LIN Peng.The effects of nutritions on the C. vulgaris growth and the content of protein[J].Journal of Dalian University,2002,23(6):12-16. |
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| Authors: | YU Yuan LIU Yan YANG Hai-bo ZHANG Xin-hua LI Ying-min LU Fu-Rong LIN Peng |
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| Abstract: | Protein and glyooprotein in microalgae have many important biological activities and physiolgical functions. Under the conditions of 24 - 26 ℃ and natural light, the relationship between the growth of C. vulgaris and the accumulation of protein was investigated, and then the effects of nutritions on the C. vulgaris growth and on the contents of protein were studied by means of orthogonal design test. The results have showed that the synthesis and accumulation of protein in C. vulgaris occur in the stationary phase. The Concentration and optimal combination of nutritions depent on the experimental intention. For the C. vulgaris growth, the optimal combination of nutritions is C6H12O6: 2g/L, KNO3: 0.8g/L, NaH2PO4: 0.2g/L, the highest cell density is 6.46×107 cells/L culture; and for the contents of protein, that of nutritions is C6H12O6: 6g/L, KNO3: 1.6g/L, NaH2PO4: 0.2 g/L. The correspond contents of protein is 655.4 mg/g dry weight. The experiment results provide the laboratory foundation and precondition for further optimally culturing C. vulgaris in great scale and further obtaining the important monoforms of protein from C. vulgaris. |
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| Keywords: | C vulgaris nutrition protein orthogonal design |
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