| 间接血凝试验(IHA)检测肝片形吸虫病的研究 |
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| 引用本文： | 倪兆朝,周仁贵,柏庆荣,陶建平.间接血凝试验(IHA)检测肝片形吸虫病的研究[J].安徽科技学院学报,2006,20(3):5-7. |
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| 作者姓名： | 倪兆朝 周仁贵 柏庆荣 陶建平 |
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| 作者单位： | 江苏省高邮市畜牧兽医站,江苏,高邮,225600;江苏省仪征市马集镇畜牧兽医站,江苏,仪征,211400;扬州大学兽医学院,江苏,扬州,225009 |
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| 基金项目： | 肉类食品寄生虫检测方法及标准的研究(科技部公益性项目) |
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| 摘 要： | 以肝片形吸虫成虫虫体制备的纯化抗原为诊断液,采用间接血凝试验(IHA)检测了40只山羊和160头牛血清,并与粪便检查法作平行试验。结果显示肝片形吸虫阳性检出率,IHA法为12.00%,粪便检查法为9.00%。与粪检结果相比,总的血清阳性符合率为62.50%,其中牛为57.14%,山羊为100.00%;总的血清阴性符合率为98.29%,其中牛为97.85%,山羊为100.00%。结论:IHA适合于大规模的血清流行病学调查,可以快速的筛选出大量的阴性样品。
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| 关 键 词： | 肝片形吸虫 牛 山羊 间接血凝试验 检测 |
| 文章编号： | 1672-3589（2006）03-0005-03 |
| 收稿时间： | 2006-03-18 |
| 修稿时间： | 2006年3月18日 |
Studies on the Diagnosis of Fascioliasis in Cattle and Goats by IHA |
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| NI Zhao-chao,ZHOU Ren-gui,BO Qing-rong,TAO Jian-ping.Studies on the Diagnosis of Fascioliasis in Cattle and Goats by IHA[J].Journal of Anhui Science and Technology University,2006,20(3):5-7. |
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| Authors: | NI Zhao-chao ZHOU Ren-gui BO Qing-rong TAO Jian-ping |
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| Institution: | 1. Station of Animal Husbandry and Veterinary Medicine of Gaoyou County,Gaoyou 225600,China; 2. Station of Animal Husbandry and Veterinary Medicine of Maji Twon,Yizheng 211400,China; 3. College of Veterinary Medicine, Yangzhou University, Yangzhou 225009,China |
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| Abstract: | A total of 200 sera samples,which the 40 samples prepared from cattle and the 160 samples from goats,were tested by IHA in which the pure antigen from adult worms of Fasciola hepatica was used as diagnostic antigens.Simultaneously the faecal eggs from the same animals were examined by the traditional method.The positive rates were 12.00 % for IHA and 9.00 % for faecal eggs,respectively.The total of positive specificities was 62.50 %,in which 57.14 % for cattle and 100.00 % for goats,respectively.The total of negative specificities was 98.29 %,in which 97.85 % for cattle and 100.00 % for goats,respectively.The results indicated that IHA could be used widely in epidemiological study of fascioliasis and rapidly found the negative samples. |
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| Keywords: | Fasciola hepatica Cattle Goat IHA Diagnosis |
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