| 厌氧消化器中甲烷古菌的分离与纯化 |
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| 引用本文： | 王彬,邓功成,苏小二.厌氧消化器中甲烷古菌的分离与纯化[J].黔南民族师范学院学报,2014,34(5):112-116. |
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| 作者姓名： | 王彬 邓功成 苏小二 |
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| 作者单位： | 1. 贵州省遵义县枫香中学,贵州遵义56112
2. 黔南民族师范学院生命科学系,贵州都匀55800 |
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| 基金项目： | 黔教高发[2012]426号黔南民族师范学院微生物学重点学科，黔南民族师范学院教学质量工程项目院教发[2011]5号 |
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| 摘 要： | 目的：探索分离纯化严格厌氧微生物的方法。方法：运用微生物学基础理论和Hungate厌氧操作系统,采用滚管分离培养方法对厌氧消化器中甲烷菌进行富集、滚管分离纯化。结果：获得甲烷菌纯培养。结论：Hungate滚管分离培养技术方法可靠,分离的效果好,投资不大,适用于各种厌氧微生物的分离和培养。
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| 关 键 词： | 甲烷古菌 Hungate除氧系统 富集 滚管分离 |
Separation and Purification of Methanoarchaea in the Anaerobic Digester |
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| Wang Bin,Deng Gong-cheng,Su Xiao-er.Separation and Purification of Methanoarchaea in the Anaerobic Digester[J].Journal of Qiannan Normal College of Nationalities,2014,34(5):112-116. |
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| Authors: | Wang Bin Deng Gong-cheng Su Xiao-er |
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| Institution: | Wang Bin , Deng Gong-cheng, Su Xiao-er (1. Fengxiang Middle School, Zunyi 56112, Guizhou , China; 2.3. Department of Life Science, Qiannan Normal College for Nationalities, Duyun 558000, Guizhou, China) |
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| Abstract: | The objective is to explore the way of separating and purifying anaerobic microorganism. This paper uses the basic theories of microbiology and the operating system of Hungate anaerobe and a- dopts roiling tube separation to enrich, roll -tube, separate and purify the methanoarchaea in the an- aerobic digester. The results show that the purely cultivated methanoarchaea is obtained. The conclu- sion demonstrates that roll tube cuhivation method is reliable, the effect of separation is good, the in- vestment is small, therefore, this technology is suitable for separation and cultivation of all kinds of an- aerobic microorganisms. |
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| Keywords: | Methanoarchaea Hungate of anaerobic operating system Enrichment Rolling tube sepa- ration |
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