Proteomic analysis of differentially expressed proteins in mice with concanavalin A-induced hepatitis |
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| Authors: | Xu-fei Tan Feng Chen Shan-shan Wu Yu Shi Dong-cheng Liu and Zhi Chen |
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| Institution: | (1) Department of Immunology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, People’s Republic of China;(2) Department of Immunology, School of Medicine, Yangtze University, Jingzhou, 434023, People’s Republic of China;(3) Institute of Organ Transplantation, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, People’s Republic of China |
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| Abstract: | Objective: To find new protein biomarkers for the detection and evaluation of liver injury and to analyze the relationship between such proteins and disease progression in concanavalin A (Con A)-induced hepatitis. Methods: Twenty-five mice were randomly divided into five groups: an untreated group, a control group injected with phosphate buffered saline (PBS), and groups with Con A-induced hepatitis evaluated at 1, 3 and 6 h. Two-dimensional gel elec-trophoresis (2-DE) and mass spectrometry (MS) were used to identify differences in protein expression among groups. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to verify the results. Results: In mice with Con A-induced hepatitis, expression levels of four proteins were increased: RIKEN, fructose bisphosphatase 1 (fbp1), ketohexokinase (khk), and Chain A of class pi glutathione S-transferase. Changes in fbp1 and khk were confirmed by qRT-PCR. Conclusion: Levels of two proteins, fbp1 and khk, are cleady up-regulated in mice with Con A-induced hepatitis. |
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| Keywords: | Concenavalin A (Con A) Liver injury Protein Two-dimensional electrophoresis (2-DE) |
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