| 皖北地区大豆根瘤菌优良抗逆性菌株的筛选 |
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| 引用本文： | 吴萍,何庆元,李正鹏,史均,祝嫦巍,盛伟.皖北地区大豆根瘤菌优良抗逆性菌株的筛选[J].安徽科技学院学报,2012,26(2):15-22. |
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| 作者姓名： | 吴萍 何庆元 李正鹏 史均 祝嫦巍 盛伟 |
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| 作者单位： | 安徽科技学院生命科学学院,安徽凤阳,233100;安徽科技学院生命科学学院,安徽凤阳,233100;安徽科技学院生命科学学院,安徽凤阳,233100;安徽科技学院生命科学学院,安徽凤阳,233100;安徽科技学院生命科学学院,安徽凤阳,233100;安徽科技学院生命科学学院,安徽凤阳,233100 |
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| 基金项目： | 安徽省教育厅自然科学基金项目，安徽科技学院生物学重点建设学科项目 |
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| 摘 要： | 从皖北不同地区采集土壤样品,利用宿主结瘤法获得大豆根瘤,并采用平板划线法分离不同地区大豆根瘤菌株,共31株,对其进行染料及化学药物抗性、耐酸碱性、生长温度范围、耐盐性、抗生素抗性等性状的研究。结果表明,从31个菌株中筛选出总体抗性水平最为突出的菌株AWCS 13-4,其表现抗染料及化学药物能力最强;在pH4-pH12条件下生长良好,具有较广的酸碱度适应范围;在50℃条件下,仍可以缓慢生长;在6.0%NaCl处理下仍能生长,显示出很强的耐盐能力;并且在供试5种抗生素的不同浓度条件下均生长良好,表现出极强耐抗生素的能力。
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| 关 键 词： | 大豆根瘤茵 抗逆性 筛选 |
Screening of Rhizobium from Soybean in North Anhni for Strong Resistance to Stresses |
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| WU Ping,HE Qing-yuan,LI Zheng-peng,SHI Jun,ZHU Chang-wei,SHENG Wei.Screening of Rhizobium from Soybean in North Anhni for Strong Resistance to Stresses[J].Journal of Anhui Science and Technology University,2012,26(2):15-22. |
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| Authors: | WU Ping HE Qing-yuan LI Zheng-peng SHI Jun ZHU Chang-wei SHENG Wei |
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| Institution: | (College of Life Science,Anhui Science and Technology University,Fengyang 233100,China) |
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| Abstract: | The soil samples were collected from different ecotyps of North Anhui.Soybean nodulations were obtained by planting host in soil.31 rhizobium strains were isolated and purified from each host.The experiments of stress resistance to chemistry medicament,dye,pH,temperature,salinity,and antibiotic on 31 strains were performed.The results indicated that the AWCS 13-4 has the highest resistance.It could grow in solution of dye and chemistry drug,between pH4 and pH12,50℃,6.0% solution of sodium chloride and different concentration of 5 sorts of antibiotics,which shows the AWCS13-4 has strongest resistance to dye and chemistry drug,wider range pH,higher temperature,high concentration of sodium chloride and broad-spectrum resistance of antibiotics. |
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| Keywords: | Soybean rhizobia Resistance to stress Screening |
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