| 高效液相色谱分析西红柿中的维生素C含量 |
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| 引用本文： | 李玉明,白洁.高效液相色谱分析西红柿中的维生素C含量[J].衡阳师范学院学报,2007,28(6):102-104. |
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| 作者姓名： | 李玉明 白洁 |
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| 作者单位： | 衡阳师范学院,化学与材料科学系,湖南,衡阳,421008 |
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| 摘 要： | 利用高效液相色谱仪对西红柿中维生素C的含量进行了分析研究。采用高效液相色谱法,以超纯水为流动相,流动相的速度为1.0ml/min,紫外检测波长为267nm,柱温在25℃的条件下,分析西红柿中维生素C的含量,及其重复性和回收率。通过线性回归具有良好的线性关系,其相关系数R2=0.9994;其回收率为98.71%~103.29%,相对标准偏差为0.79%。所建立的方法简单、快速、准确率高,适宜于西红柿中维生素C含量的测定。
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| 关 键 词： | 高效液相色谱法 西红柿 维生素C |
| 文章编号： | 1673-0313（2007）06-0102-03 |
| 收稿时间： | 2007-09-08 |
| 修稿时间： | 2007年9月8日 |
Study on the Content of Vitamin C in the Tomato by HPLC |
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| LI Yu-ming,BAI Jie.Study on the Content of Vitamin C in the Tomato by HPLC[J].journal of Hengyang Normal University,2007,28(6):102-104. |
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| Authors: | LI Yu-ming BAI Jie |
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| Institution: | Chemistry and Material Science Department, Hengyang Normal University, Hengyang Hunan 421008,China |
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| Abstract: | In this article, HPLC was performed to research the content of Vitamin C in the tomato. In this way, the content, the repeatability and the returnsratio of the Vitamin C in the tomato were analyzed under the condition of that ultra-pure water was taken as flowing, and its speed was 1.0ml/min, and the ultraviolet examination wave length was 267nm with the column tem- perature under the 25%. The good linear relations were gotten through the linear return with its correlation coefficient being R^2=0.9994. Its returns-ratio was 98.71%-103.29%. The relative standard deviation was 0. 79%. The method established was simple, fast and accurate, which will be suitable to analyze the content of the Vitamin C in the tomato. |
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| Keywords: | high performance liquid chromatography(HPLC) tomato vitamin C |
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