| Survival of the biocontrol agents Brevibacillus brevis ZJY-1 and Bacillus subtilis ZJY-116 on the spikes of barley in the field |
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| 作者姓名： | 张昕 张炳欣 张震 沈卫峰 杨庆鸿 喻景权 赵宇华 |
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| 作者单位： | Department of Plant Protection，School of Agriculture and Biotechnology，Zhejiang University，Hangzhou 310029，China，Department of Plant Protection，School of Agriculture and Biotechnology，Zhejiang University，Hangzhou 310029，China，Academy of Agriculture Science，Hangzhou 310021，China，Academy of Agriculture Science，Hangzhou 310021，China，Department of Biological Sciences，University of Wisconsin-Milwaukee，WI 53211，USA，Department of Horticulture，School of Agriculture and Biotechnology，Zhejiang University，Hangzhou 310029，China，School of Life Science，Zhejiang University，Hangzhou 310029，China |
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| 基金项目： | 国家自然科学基金，Science and Technology Committee of Zhejiang Province |
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| 摘 要： | INTRODUCTION Soil-borne pathogens, including Pythium spp. and Fusarium spp., cause significant yield losses in horticulture and agriculture crops (Mao et al., 1997). Current practices for controlling plant diseases are based largely on disease resistant crops, cultivation management in fields and application of synthetic pesticides (Elizabeth and Emmert, 1999). Biological control using antagonistic microbes to reduce the use of chemical pesticides in a system of integrated plantdisease …
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| 关 键 词： | 大麦 绿色荧光蛋白质 微生物病害 FHB 杆状菌 生物电控制 |
Survival of the biocontrol agents Brevibacillus brevis ZJY-1 and Bacillus subtilis ZJY-116 on the spikes of barley in the field |
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| ZHANG Xin,ZHANG Bing-xin,ZHANG Zhen,SHEN Wei-feng,YANG Ching-hong,YU Jing-quan,ZHAO Yu-hua.Survival of the biocontrol agents Brevibacillus brevis ZJY-1 and Bacillus subtilis ZJY-116 on the spikes of barley in the field[J].Journal of Zhejiang University Science,2005,6(8):770-777. |
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| Authors: | ZHANG Xin ZHANG Bing-xin ZHANG Zhen SHEN Wei-feng YANG Ching-hong YU Jing-quan ZHAO Yu-hua |
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| Abstract: | Fusarium head blight (FHB) caused by Fusarium graminearum is a devastating disease that results in extensive yield losses to wheat and barley. A green fluorescent protein (GFP) expressing plasmid pRP22-GFP was constructed for monitoring the colonization of two biocontrol agents, Brevibacillus brevis ZJY-1 and Bacillus subtilis ZJY-116, on the spikes of barley and their effect on suppression of FHB. Survival and colonization of the Brevibacillus brevis ZJY- 1 and Bacillus subtilis ZJY- 116 strains on spikes of barley were observed by tracking the bacterial transformants with GFP expression. Our field study revealed that plasmid pRP22-GFP was stably maintained in the bacterial strains without selective pressure. The retrieved GFP-tagged strains showed that the bacterial population fluctuation accorded with that of the rain events. Furthermore, both biocontrol strains gave significant protection against FHB on spikes of barley in fields. The greater suppression of barley FHB disease was resulted from the treatment of barley spikes with biocontrol agents before inoculation with F. graminearum. |
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| Keywords: | GFP Survival Brevibacillus brevis and Bacillus subtilis Spikes Barley Biocontrol |
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