A novel technique of three-dimensional reconstruction segmentation and analysis for sliced images of biological tissues |
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| Authors: | Li Jing Zhao Hai-yan Ruan Xing-yun Xu Yong-qing Meng Wei-zheng Li Kun-peng Zhang Jing-qiang |
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| Institution: | (1) Department of Equipments, Kunming General Hospital, Kunming, 650032, China;(2) State Key Laboratory for Biocontrol, School of Life Science, Sun Yat-sen University, Guangzhou, 510275, China;(3) Department of Ophthalmology, Yunnan Province People’s Hospital, Kunming, 650032, China;(4) Department of Orthopaedics, Kunming General Hospital, Kunming, 650032, China |
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| Abstract: | A novel technique of three-dimensional (3D) reconstruction, segmentation, display and analysis of series slices of images including microscopic wide field optical sectioning by deconvolution method, cryo-electron microscope slices by Fourier-Bessel synthesis and electron tomography (ET), and a series of computed tomography (CT) was developed to perform simultaneous measurement on the structure and function of biomedical samples. The paper presents the 3D reconstruction segmentation display and analysis results of pollen spore, chaperonin, virus, head, cervical bone, tibia and carpus. At the same time, it also puts forward some potential applications of the new technique in the biomedical realm. |
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| Keywords: | Sliced images 3D reconstruction and analysis 3D segmentation Chaperonin Virus |
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