| 水稻珠心细胞的超微结构及酸性磷酸酶活性的动态变化 |
| |
| 引用本文： | 欧阳学智,李宝健,张志宇.水稻珠心细胞的超微结构及酸性磷酸酶活性的动态变化[J].中山大学学报论丛,1989(4). |
| |
| 作者姓名： | 欧阳学智 李宝健 张志宇 |
| |
| 作者单位： | 仲恺农业技术学院 |
| |
| 基金项目： | 国家‘863’资助项目 |
| |
| 摘 要： | 水稻在大孢子发生期间,珠心细胞保留了较多的分生组织细胞的特征:其细胞形态规则,细胞质浓厚,富含核糖体。核糖体游离于细胞质中,或附着于内质网及核外膜上。酸性磷酸酶的反应产物主要位于小液泡中,偶见于高尔基潴泡及小泡中。随着胚囊的分化,在珠心的内层出现一系列复杂的变化:①在细胞的液泡化过程中,频繁并持续地发生液泡对细胞质组分的吞噬,导致大液泡的形成,其中有很多的酸性磷酸酶反应产物。大液泡的进一步扩大和继后的破裂,引起细胞的解体;②粗面内质网槽库彭大并对细胞质进行分隔与吞噬,在核周腔内出现膜束縛内含物;③质膜与细胞壁分离,二者之间有大量的小泡及酸性磷酸酶反应产物。最后珠心细胞的原生质强烈收缩,转化为无结构的电子致密的残迹。
|
| 关 键 词： | 水韬 珠心细胞 超微结构 酸性磷酸酶 细胞化学 |
Dynamic Changes of the Ultrastructure and Acid Phosphatase Activity in Rice Nucellar Cells |
| |
| Ouyang Xuezhi Li Baojian Zhang Zhiyu.Dynamic Changes of the Ultrastructure and Acid Phosphatase Activity in Rice Nucellar Cells[J].Supplement to the Journal of Sun Yatsen University,1989(4). |
| |
| Authors: | Ouyang Xuezhi Li Baojian Zhang Zhiyu |
| |
| Institution: | Ouyang Xuezhi Li Baojian Zhang Zhiyu |
| |
| Abstract: | The dynamic changes in the ultrastructure and acid phosphatase activity of rice(Oryza sativa)nucellar cells were described during the processe of megasporogenesis and embryo sac formation. During the megasporogenesis of rice(Oryze sativa), nucellar cells retain more characteristics of meristematic cells. They are regular in form, highly cytoplasmic and rich in ribosomes, either suspended in the cytoplasm or attached to the endoplasmic reticulum and on the outer nuclear membrane. Reaction product of acid phosphatase is localized primarily in the small vacuoles, and occasionally in the dictyosome cisternae and vesicles of the golgi body. With the differentiation of embryo sac, a series of complicated changes occur in the inner layers of the nucellus. Vacuoles encapsulate, frequently and continuously, portions of cytoplasm containing ribosome, plastids, mitochondria and vesicles, resulting in the formation of larger vacuoles with large amount of reaction product of acid phosphatase. The further enlargement ansubsequent rupture of the large vacuoles cause the destruction of the nucellar cells. The second change include the dilation of rough endoplasmic reticulum cisternae and their engulfing and sequestering cytoplasmic components, and the occurrence of the membrance-bound contents within the nuclear envelope. The third change was the seperation of the plasmalemma from the cell wall and the subsequent formation of the large space between the plasmallemma and the cell wall, in which numerous small vacuoles and heavy reaction product of acid phosphatase were observed. Finally protoplasms of the nucellar cells shrinks dramatically and convert to disorganized electron-dense debris. |
| |
| Keywords: | rice(Oryza Sativa) nucellar cells ultrastructure acid phosphatase cytochemistry |
| 本文献已被 CNKI 等数据库收录！ |
|
