| 甜柿品种"富有"组织培养技术研究 |
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| 引用本文： | 刘恺,贾春凤.甜柿品种"富有"组织培养技术研究[J].保定师专学报,2007,20(2):51-52,62. |
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| 作者姓名： | 刘恺 贾春凤 |
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| 作者单位： | 保定学院,化学系,河北,保定,071000,保定学院,综合理科系,河北,保定,071000 |
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| 摘 要： | 较系统地对甜柿(Diospyros kaki Thunb.)品种"富有"柿组培苗的继代增殖、叶片不定芽再生和生根的培养条件进行研究.试验结果表明:"富有"柿的最佳长期继代培养基为MS+ZT2.0 mg/L+IAA 0.1 mg/L;高效不定芽再生体系在培养基MS(1/2N)+TDZ 0.4 mg/L+ZT1.0 mg/L培养30 d之后,转入培养基MS(1/2 N)+IAA 0.1 mg/L+ZT 2.0 mg/L中,其分化率达到90.3%以上,外植体平均芽数为4.6;生根选取在培养基1/2 MS+IBA 1.0 mg/L培养基中暗培养8 d,生根率为93.1%.
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| 关 键 词： | 甜柿 "富有" 组培快繁 不定芽再生 |
| 文章编号： | 1008-4584(2007)02-0051-03 |
| 修稿时间： | 2006年12月20日 |
Study on Tissue Culture "Fuyu" Persimmon (Diospyros kaki Thunb.) |
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| LIU Kai,JIA Chun-feng.Study on Tissue Culture "Fuyu" Persimmon (Diospyros kaki Thunb.)[J].Journal of Baoding Teachers College,2007,20(2):51-52,62. |
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| Authors: | LIU Kai JIA Chun-feng |
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| Institution: | 1. Department of Chemistry, Baoding University, Baoding 071000, China; 2. Department of Comprehensive Science, Baoding University, Baoding 071000, China |
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| Abstract: | Study was carried out on effection of "Fuyu"on micropropagation,shoot regeneration and rooting.The result indicated that: MS medium supplementing ZT 2.0 mg/L and IAA 0.1 mg/L was the best medium for micropropagation.The best regeneration was achieved in MS(1/2N)+TDZ 0.4 mg/L +ZT 1.0 mg/L.After 30 days culture,the callus were subdivided and transferred to MS(1/2N)medium supplemented with ZT 2.0 mg/L and IAA 0.1 mg/L.Regeneration rate was 90.3 %,average number of bud of each explant was 4.6.1/2MS medium supplementing IBA 1.0 mg/L was the best medium for inducing rooting by culturing 8 days under dark,and the rooting rate was 93.1 %. |
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| Keywords: | Persimmon "Fuyu" micropropagation plant regeneration |
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