| 蜜蜂转基因技术 |
| |
| 引用本文： | 张巧利,孙亮先,郭冬生,胥保华.蜜蜂转基因技术[J].泉州师范学院学报,2005,23(4):94-99. |
| |
| 作者姓名： | 张巧利 孙亮先 郭冬生 胥保华 |
| |
| 作者单位： | 1. 泉州师范学院,模式生物研究中心,福建,泉州,362000
2. 山东农业大学,动物科技学院,山东,泰安,271018 |
| |
| 基金项目： | 国家自然科学基金项目（30300183）;山东省博士基金项目（2004BS06014） |
| |
| 摘 要： | 简述了建立蜜蜂转基因技术体系的方法和筛选标记，以显微注射法将改造过的piggyBac、mariner等转座子转基因载体导入受精卵是蜜蜂转基因工作的最佳候选方案，精子介导法和病毒载体法也值得尝试，增强型荧光蛋白（EGFP）基因是转基因蜜蜂的最佳筛选标记，并对蜜蜂转基因技术体系在基因功能研究和生物反应器的开发等方面的应用前景进行了探讨。
|
| 关 键 词： | 蜜蜂 转基因 载体 显微注射 转座子 精子 |
| 文章编号： | 1009-8224(2005)04-0094-06 |
| 收稿时间： | 04 9 2005 12:00AM |
| 修稿时间： | 2005年4月9日 |
Advances in the Study of Transgenic Honeybee |
| |
| ZHANG Qiao-li,SUN Liang-xian,GUO Dong-sheng,XU Bao-hua.Advances in the Study of Transgenic Honeybee[J].Journal of Quanzhou Normal College,2005,23(4):94-99. |
| |
| Authors: | ZHANG Qiao-li SUN Liang-xian GUO Dong-sheng XU Bao-hua |
| |
| Institution: | 1. Model Organism Research Center, Quanzhou Normal University,Fujian 362000, China; 2. Animal College of Science and Technology,Shandong Agricultural University,Taian 271018,China |
| |
| Abstract: | In this review, we discussed the approaches for establishing honeybee germline transformation system as well as the suitable marker for screening transgenic honeybee. Among the insect germline transformation systems ,injecting the transgenic vector based on transposon piggyback and mariner into fertilized egg by microinjection was preferred in establishing honeybee germline transformation system. Sperm and certain reverse virus mediated gene transfer were also possible for honeybee. And the EGFP marker was consider suitable for screening transgenic honeybee. We also discussed the potential usage of honeybee transformation system in investigating function of honeybee gene and developing a new type of bioreactor. |
| |
| Keywords: | honeybee transformation vector microinjection transposon sperm |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
