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荧光分光光度法测定丁胺卡那霉素含量
引用本文:马春华,陈培珍.荧光分光光度法测定丁胺卡那霉素含量[J].南平师专学报,2008,27(5):32-34.
作者姓名:马春华  陈培珍
作者单位:武夷学院绿色化工技术重点实验室化学与环境工程系
基金项目:南平市科技局资助项目:(N2005202)
摘    要:根据伯胺类和邻苯二甲醛及巯基乙醇生成异吲哚取代生物这一灵敏的反应,利用荧光分光光度法测丁胺卡那霉素含量。结果,卡那霉素含量在0~20.00ug·ml^-1呈线性关系,检测线为0.034ug·ml^-1,检测药品回收率为98.14%~100.49%。

关 键 词:卡那霉素  荧光分光光度

Fluorescence Spectrophotometry for Determination Amikacin
MA Chunhua,CHEN Peizhen.Fluorescence Spectrophotometry for Determination Amikacin[J].Journal of Nanping Teachers College,2008,27(5):32-34.
Authors:MA Chunhua  CHEN Peizhen
Institution:(Chemistry and Environment Engineering Department of Wuyi University,Wuyishan,Fujian 354300)
Abstract:Amikacin. OPA and mercaptoethanol can react to form indole derivatives, thus set up a new method to detect amikacin. Fluorescence intensity and concentration present good linear relationship when the concentration of kanamycin was 0-20.00ug· ml^-1 ,the detection limit was 0. 034ug· ml^-1, The method has been applied to analysis the content of commercial amikacin injection with recovery rate of 98.14%-100. 49% .
Keywords:amikacin  fluorescence  spectrophotometry
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