| 心肌SarcKATP通道kir6.2亚基在运动预适应心肌保护效应中变化的研究 |
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| 引用本文： | 王凯,潘珊珊,王庆棠.心肌SarcKATP通道kir6.2亚基在运动预适应心肌保护效应中变化的研究[J].体育科学,2012,32(4):60-66,83. |
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| 作者姓名： | 王凯 潘珊珊 王庆棠 |
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| 作者单位： | 上海体育学院运动科学学院,上海,200438 |
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| 基金项目： | 国家自然科学基金资助项目，上海市科学技术委员会资助项目 |
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| 摘 要： | 目的:探讨心肌SarcKATP通道kir6.2在运动预适应心肌保护效应中的变化以及与PKC的关系。方法:SD大鼠分对照组(C组)、力竭运动组(EE组)、运动预适应组(EP组)、运动预适应+力竭运动组(EP+EE组)、PKC阻断剂+运动预适应组(CHE+EP)和PKC阻断剂+运动预适应+力竭运动组(CHE+EP+EE组)。一次大强度间歇跑台运动建立运动预适应模型,力竭跑台运动致大鼠心肌损伤。用原位杂交和实时荧光定量PCR法检测kir6.2mRNA变化,用免疫荧光和免疫印迹法检测kir6.2蛋白变化。结果:与C组比,EE组和EP组kir6.2mRNA无明显变化,而EE组kir6.2蛋白明显升高,EP组kir6.2蛋白明显下降。与EE组比,EP+EE组kir6.2mRNA无明显变化,kir6.2蛋白明显下降。与EP组比,CHE+EP组kir6.2mRNA明显降低,kir6.2蛋白明显升高。与EP+EE组比,CHE+EP+EE组kir6.2mRNA和kir6.2蛋白无明显变化。结论:在EP诱导的保护效应中,心肌SarcKATP通道kir6.2mRNA水平未发生明显变化,而kir6.2蛋白水平明显降低,提示,心肌SarcKATP通道通过kir6.2蛋白水平的降低介导EP诱导的心肌保护效应。PKC对心肌SarcKATP通道的表达具有调控作用。
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| 关 键 词： | SarcKATP通道 kir6.2亚基 运动预适应 心肌保护 力竭运动 心肌损伤 鼠 动物实验 |
Research on Changes of the Pore-Forming Subunit kit6.2 of Myocardial Sarcolemmal ATP-Sensitive Potassium Channels in Myocardial Protection of Exercise Preconditioning |
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| WANG Kai
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PAN Shan-shan
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WANG Qing-tang.Research on Changes of the Pore-Forming Subunit kit6.2 of Myocardial Sarcolemmal ATP-Sensitive Potassium Channels in Myocardial Protection of Exercise Preconditioning[J].China Sport Science,2012,32(4):60-66,83. |
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| Authors: | WANG Kai PAN Shan-shan WANG Qing-tang |
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| Institution: | School of Sports Science,Shanghai University of Sport,Shanghai 200438,China. |
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| Abstract: | Objective:To study the changes of the Pore-Forming Subunit kir6.2 of myocardial sarcolemmal ATP-sensitive potassium(SarcKATP) channels in myocardial protection of exercise preconditioning(EP) and the relations with protein kianse C(PKC).Methods:SD rats were divided into control group(C),EE group,EP group,EP+EE group,CHE+EP group and CHE+EP+EE group.By using once heavy intensity interval treadmill running,this paper establishes EP model,and exhaustive exercise on treadmill to induce rats myocardial injury.In-situ hybridization and real-time fluorescence quantitative PCR methods were used to detect changes of kir6.2 mRNA.Immunofluorescence and westten blotting methods were used to detect changes of kir6.2 protein.Results:Compared with group C,there is no significant change of kir6.2 mRNA in group EE and EP,while kir6.2 protein significantly increased in group EE and significantly decreased in group EP.Compared with group EE,no significant changes of kir6.2 mRNA in group EP+EE,kir6.2 protein in group EP+EE significantly decreased.Compared with group EP,kir6.2 mRNA significantly decreased in group CHE+EP,kir6.2 protein significantly increased in group CHE+EP.Compared with group EP+EE,there is no significant changes of kir6.2 mRNA and kir6.2 protein in group CHE+EP+EE.Conclusion:In myocardial protection induced by EP,kir6.2 mRNA did not changed significantly,while kir6.2 protein decreased significantly,which demonstrated myocardial SarcKATP channels mediated myocardial protection induced by EP through kir6.2 protein decreased.PKC can regulate expression of myocardial SarcKATP channels. |
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| Keywords: | Sarcolemmal ATP-sensitive potassium channels the pore-forming subunit kir6 2 exercise preconditioning myocardial protection exhaustive exercise myocardial injury rat animal experiment |
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