| 力竭运动应激对小鼠T淋巴细胞钾离子通道Kv1.3的调节作用 |
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| 引用本文： | 傅涛,王丽春,郝选明.力竭运动应激对小鼠T淋巴细胞钾离子通道Kv1.3的调节作用[J].体育学刊,2010,17(5). |
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| 作者姓名： | 傅涛 王丽春 郝选明 |
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| 作者单位： | 1. 华南师范大学,体育科学学院,广东,广州,510006
2. 中国医学科学院中国协和医科大学医学生物学研究所病毒免疫室,云南,昆明,650118 |
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| 摘 要： | 为了观察力竭运动应激后对小鼠外周血T淋巴细胞钾离子通道表达以及对细胞调节性容积减少(regulatory volume decrease,RVD)的影响,探讨力竭运动应激导致的免疫抑制的可能机制.复制小鼠力竭模型后从外周血中分离出T淋巴细胞,测定其淋巴细胞转化功能,采用RT-PCR、Western blot分别从基因和蛋白质水平揭示力竭运动应激对小鼠外周血T细胞Kv1.3表达的调节作用,采用细胞成像系统测定T淋巴细胞在低渗透压时细胞容积的变化.结果显示力竭运动应激小鼠T淋巴细胞功能受抑制(P＜0.05),小鼠外周血T淋巴细胞Kv1.3 mRNA和蛋白质表达水平明显下调(P＜0.05),力竭运动应激会影响RVD的调节能力.结果说明,力竭运动应激导致小鼠外周血T淋巴细胞Kv 1.3 mRNA和蛋白质表达水平明显下调,RVD调节能力下降,提示力竭运动应激条件下免疫功能的抑制可能与钾通道有关.
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| 关 键 词： | 运动生物化学 Kv1.3免疫抑制 力竭运动应激 |
Functions of exhausting exercise stress in regulating the potassium ion channels (Kv1.3) of T lymphocytes of mice |
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| FU Tao,WANG Li-chun,HAO Xuan-ming.Functions of exhausting exercise stress in regulating the potassium ion channels (Kv1.3) of T lymphocytes of mice[J].Journal of Physical Education,2010,17(5). |
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| Authors: | FU Tao WANG Li-chun HAO Xuan-ming |
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| Institution: | FU Tao1,WANG Li-chun2,HAO Xuan-ming1(1.School of Physical Education,South China Normal University,Guangzhou 510006,China,2.Peking Union Medical College,Institute of Medical Biology,Chinese Academy of Medical Sciences,Kunming 680118,China) |
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| Abstract: | In order to observe the effects of exhausting exercise stress on the expression of the potassium ion channels of T lymphocytes in the peripheral blood of mice and the regulatory volume decrease(RVD) of cells,and to probe into a possible mechanism of immunosuppression caused by exhausting exercise stress,the authors separated T lymphocytes from the peripheral blood after having duplicated the mouse exhausting model,measured its lym-phocyte transformation functions,revealed the functions of exhausting exercis... |
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| Keywords: | exercise biochemistry Kv1 3 immunosuppression exhausting exercise stress |
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