| 人生长激素的单克隆抗体放射免疫分析法和免疫放射分析法 |
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| 引用本文： | 周湖军,张少华,钱卫平.人生长激素的单克隆抗体放射免疫分析法和免疫放射分析法[J].科技通报,1991(3). |
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| 作者姓名： | 周湖军 张少华 钱卫平 |
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| 作者单位： | 南京大学 南京210008(周湖军)，南京农业大学畜牧系 南京210014(张少华)，东南大学生物医学工程系 南京210018(钱卫平) |
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| 摘 要： | 利用抗HGH单克隆抗体建立了HGH的RIA和IRMA。方法学鉴定表明,这两种测定方法的灵敏度高(RIA为1.2ng/ml,IRMA为2ng/ml)、特异性强(与HPL及HPRL均无交叉反应)、重复性好。同时,还将这两种方法与HGH常规抗血清RIA进行了比较。
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| 关 键 词： | 人生长激素 放射免疫分析法 免疫放射分析法 单克隆抗体 人胎盘催乳素 人催乳素 常规抗血清 |
Radioimmunoassay and immunoradiometricassay of human growth hormone using monoclonal antibodies |
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| Zhou Huiun,Nanjing UniversityZhang Shaohua.Radioimmunoassay and immunoradiometricassay of human growth hormone using monoclonal antibodies[J].Bulletin of Science and Technology,1991(3). |
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| Authors: | Zhou Huiun Nanjing UniversityZhang Shaohua |
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| Institution: | Zhou Huiun,Nanjing UniversityZhang Shaohua,Department of Animal Science,Nanjing Agricultural UniversityOian Weiping,Department of Biomedical Engineering,Southeast University,Nanjing |
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| Abstract: | A mixture of 3 monoclonal antibodies(McAbs)against human growth hor- mone(HGH)has been employed successfully to establish a radioimmunoassay (RIA)for measurement of serum HGH levels. A new one-step, two-site im- munoradiometricassay(IRMA)has been developed by using McAb-1 as the solid- phase antibody and McAb-4 as the labeled antibody. There are two non-over- lapping antigenic sites for McAb-1 and McAb-4. The results indicate that the two immunoassays are highly sensitive (RIA. 1.2ng/ml; IRMA: 2ng/ml), strongly specific(no cross-reactivity with human placental lactogen and human prolactin)and excellent repeated, and these McAbs are useful in RIA and IRMA of HGH in clinical diagnosis. Finally, the two methods are compared with the RIA using polyclonal antibodies. |
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| Keywords: | radioimmunoassay(RIA) immunoradiometricassay(IRMA) human growth hormone( HGH ) monoclonal antibody ( McAb ) |
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