| 碱性蛋白酶制备纳米珍珠粉工艺优化 |
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| 引用本文： | 夏潇潇,张靖华,高娅华,陈静怡,张洁.碱性蛋白酶制备纳米珍珠粉工艺优化[J].合肥联合大学学报,2013(1):67-69,73. |
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| 作者姓名： | 夏潇潇 张靖华 高娅华 陈静怡 张洁 |
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| 作者单位： | [1]合肥学院生物与环境工程系,合肥230601 [2]安徽华伊美科技企业集团研发部,合肥230088 |
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| 基金项目： | 安徽省教育厅自然科学重点项目（KJ2011A246）基金资助. |
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| 摘 要： | 使用碱性蛋白酶处理，考察摇床转速、酶解时间和加酶量对纳米级珍珠粉得率的影响．试验结果表明：10g珍珠粉加入50mL去离子水，在摇床转速为180r／rain、酶解时间为8h、酶用量为0．5g时，纳米级珍珠粉得率为95．2％．凯式定氮实验表明：酶解前后珍珠粉蛋白质的含量分别为24．132％和22．010％，酶解后仅相对减少了0．088．
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| 关 键 词： | 酶解 珍珠粉 纳米 |
Optimization Process of Nanometer Pearl Powder Preparation by Enzyme Digestion Using Alkali Protease |
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| Authors: | XIA Xiao-xiao ZHANG Jing-hua GAO Ya-hua CHEN Jing-yi ZHANG Jie |
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| Institution: | 1. Department of Biological and Etlvironmental Engineering, Hefei University, Hefei 230601 ; 2. R＆D department, Anhui Huayimei Cosmetics Co. Ltd. , Hefei 230088, China) |
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| Abstract: | The yield of nanometer pearl powder was used as an evaluation index, and the effects of the shaker speed, enzymatic hydrolysis time and enzyme dosage were studied. The nanometer pearl powder with 95.2% yield was obtained under the conditions of shaker speed 180 r/rain, enzymatic hydrolysis time 8 h and enzyme dosage 0.5g. The protein content of pearl powder before and after enzymatic hydrolysis was 24. 132% and 22. 010%, respectively by Kjeldahl method, and the relative reduction was only 0.088. |
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| Keywords: | enzymatic hydrolysis pearl powder nanometer |
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