Isolation and identification of Sclerotinia stem rot causal pathogen in Arabidopsis thaliana |
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| Authors: | Wang Ai-Rong Lin Wen-Wei Chen Xiao-Ting Lu Guo-Dong Zhou Jie Wang Zong-Hua |
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| Institution: | [1]Computer and Information College, Fujian Agriculture and Forestry University, Fuzhou 350002, China [2]Ministry of Education Key Laboratory of Biopesticide and Chemical Biology, Fujian Agriculture and Forestry University, Fuzhou 350002, China |
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| Abstract: | A new stem rot disease is found to occur naturally on Arabidopsis plants in greenhouses of Fuzhou, China. In order to identify its pathogen, we conducted a series of fimgal isolation and purification, plant reinoculation, and ascus and ascospore induction from the sclerotia. The isolate caused typical water-soaked lesions after reinoeulation and produced sclerotia both on Arabidopsis plants and culture medium plates, and the sclerotia could be induced to produce discal apothecia and 8 binucleate ascospores per ascus. These disease symptom and fungal morphology data revealed that the fungus Sclerotinia sclerotiorum (Lib.) de Bary was the pathogen for Arabidopsis stem rot. To confirm this, we further amplified its large subunit ribosomal DNA (LSU rDNA) by polymerase chain reaction (PCR), and compared the sequence with the known LSU rDNA sequences in GenBank. The results show that the sequence shares the highest identities with the LSU rDNAs of different S. sclerotiorum strains. Taking all these data together, we concluded that the fungus that caused the Arabidopsis stem rot is S. sclerotiorum (Lib.) de Bary. This is the first report that Arabidopsis is naturally infected by S. sclerotiorum. |
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| Keywords: | Sclerotinia sclerotiorum Arabidopsis thaliana Large subunit ribosomal DNA (LSU rDNA) Systematic classification |
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