| 花生苹果酸脱氢酶基因的克隆及其蛋白序列分析 |
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| 引用本文： | 汪巧英,;应濠泽,;余佳宁,;祝锦晶,;徐自力,;杜照奎.花生苹果酸脱氢酶基因的克隆及其蛋白序列分析[J].台州学院学报,2014(6):38-44. |
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| 作者姓名： | 汪巧英 ;应濠泽 ;余佳宁 ;祝锦晶 ;徐自力 ;杜照奎 |
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| 作者单位： | [1]台州学院生命科学学院,浙江台州318000; [2]浙江省植物进化生态学与保护重点实验室,浙江台州318000; [3]台州学院生态研究所,浙江台州318000 |
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| 基金项目： | 浙江省教育厅科研项目（Y201223322）; 2014年浙江省大学生科技创新活动计划（新苗人才计划）（2014R428013） |
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| 摘 要： | 根据已知植物的苹果酸脱氢酶基因序列设计简并引物,采用RT-PCR技术从花生叶片中克隆得到苹果酸脱氢酶基因,命名为Ah MDH,Genbank登录号为KF499119,该基因编码区长度为1071bp,编码356个氨基酸。序列比对结果表明,Ah MDH编码蛋白与大豆、蒺藜苜蓿和豌豆等具有较高的相似性。
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| 关 键 词： | 花生 苹果酸脱氢酶 克隆 序列分析 |
Cloning and Sequence Analysis of Malate Dehydrogenase Gene from Arachis hypogaea L. |
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| Institution: | XU Zi-li,DU Zhao-kui (1. School of Life Sciences, Taizhou University, Taizhou 318000, China; 2. Zhejiang Provincial Key Laboratory of Plant Evolutionary Ecology and Conservation, Taizhou 318000, China; 3.Institute of Ecology, Taizhou University, Taizhou 318000, China) |
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| Abstract: | In this paper, degenerate primers were designed according to reported malate dehyrogenase gene sequences. The coding sequence of malate dehyrogenase gene（Ah MDH） numbered KF499119 was amplified by RT-PCR from c DNA of Arachis hypogaea L. The sequence of Ah MDH is 1071 bp in length encoding 356 amino acid. Multiple sequence alignment analysis showed that A. hypogaea, Glycine max, Medicago truncatula and Pisum sativum share high homology. |
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| Keywords: | Arachis hypogaea malate dehyrogenase gene cloning sequence analysis |
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