| 不同负荷训练对大鼠骨骼肌细胞膜自由基代谢、膜流动性及Na~+-K~+-ATP酶活性的影响 |
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| 引用本文： | 潘玮敏,杨建昌.不同负荷训练对大鼠骨骼肌细胞膜自由基代谢、膜流动性及Na~+-K~+-ATP酶活性的影响[J].西安体育学院学报,2004,21(3):51-53,57. |
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| 作者姓名： | 潘玮敏 杨建昌 |
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| 作者单位： | 西安体育学院,运动人体科学系,陕西,西安,710068 |
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| 摘 要： | 探讨不同负荷游泳训练对大鼠骨骼肌细胞膜结构和功能的影响。分别测试不同负荷下膜的抗氧化酶SOD、膜脂质过氧化产物MDA、膜荧光偏振度(P)及膜Na+-K+-ATP酶活性的变化。与对照组相比,小负荷训练组骨骼肌细胞膜P值降低,膜SOD活性、Na+-K+-ATP酶活性提高,膜上MDA含量则降低;大负荷训练组较小负荷组肌膜P值增加,膜SOD活性、Na+-K+-ATP酶活性下降。适宜负荷训练可以提高骨骼肌细胞膜的抗氧化能力,改善膜脂的流动性,使膜Na+-K+-ATP酶活性增强,而过大负荷训练则使骨骼肌细胞膜的抗氧化能力、膜脂的流动性及膜酶活性降低。
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| 关 键 词： | 运动负荷 骨骼肌细胞膜 自由基 膜流动性 酶活性 |
| 文章编号： | 1001-747X(2004)03-0051-03 |
Effects of Endurance Training With Different Intensities on Free Radicals Metabolism,Membrane Fluidity and Na+-K+-ATPase Activity of Skeletal Muscle Cell Membrane in Rats |
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| PAN Wei-min,YANG Jian-chang.Effects of Endurance Training With Different Intensities on Free Radicals Metabolism,Membrane Fluidity and Na+-K+-ATPase Activity of Skeletal Muscle Cell Membrane in Rats[J].Journal of Xi'an Institute of Physical Education,2004,21(3):51-53,57. |
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| Authors: | PAN Wei-min YANG Jian-chang |
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| Abstract: | To investigate the structure and function changes of skeletal muscle cell membrane after different intensity endurance training. MDA content, SOD、 Na~+-K~+-ATPase activity, degree of fluorescence polarization (P)of skeletal muscle cell membrane were measured respectively.The results showed that SOD、 Na~+-K~+-ATPase activity and the membrane fluidity increased significantly in lower intensity group, the MDA content decreased; compared lower intensity group, SOD、 Na~+-K~+-ATPase activity and membrane fluidity in high intensity group decreased significantly. After suitable intensity training, the anti-oxidation capabilities of skeletal muscle cell membrane were enhanced ,the membrane fluidity and the Na~+-K~+-ATPase activity were improved. |
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| Keywords: | exercise intensity skeletal muscle cell membrane free radicals membrane fluidity enzyme activity |
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