| 反相胶束介质对过氧化物模拟酶催化荧光反应的影响 |
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| 引用本文： | 王全林.反相胶束介质对过氧化物模拟酶催化荧光反应的影响[J].宁夏师范学院学报,2000,21(6):25-29. |
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| 作者姓名： | 王全林 |
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| 作者单位： | 固原师专 化学系 |
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| 摘 要： | 荧光法研究了反相胶束介质对过氧化物模拟酶催化过氧化氢与邻苯二胺反应的影响。结果显示,反相胶束介质对模拟酶活性及荧光产物均具有增敏作用,对酶促反应最适宜酸度范围有所增大。另外,在反相胶束介质中,三种模拟酶的活性顺序为:Hb>>βCD—Hemin>Hemin。
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| 关 键 词： | 反相胶束 过氧化物模拟酶 催化荧光反应 增敏 酶促反应动力学 核含水量 荧光光谱 酶活性 |
The Effect of Reversed Micell on the Mimetic peroxidase-Catalyzed Fluorogenic Reaction |
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| WANG Quan-Lin.The Effect of Reversed Micell on the Mimetic peroxidase-Catalyzed Fluorogenic Reaction[J].Journal of Ningxia Teachers College,2000,21(6):25-29. |
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| Authors: | WANG Quan-Lin |
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| Abstract: | The effect of the AOT/n-octane on the mimetic peroxidase-Catalyzed fluorogenic reaction between OPDA and H2O2 were investigated. The results indicated that the activity of mimetic peroxidase and the fluorescence of the product were enhanced by AOT/n-octane, and the optimal pH range of peroxidase-Catalyzed reaction was increased by AOT/n-octane. In addition, as is evidence on from the resuts of study, the catalytic activity sequence of mimetic peroxidases is;Hb>CD-Hemin > Hemin. |
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| Keywords: | Reversed micelles Mimetic peroxidase catalyzed fluorogenic reaction |
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