| 蛋壳作载体固定化脂肪酶 |
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| 引用本文： | 刘新喜,彭立凤.蛋壳作载体固定化脂肪酶[J].宁夏师范学院学报,2000,21(6):21-24. |
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| 作者姓名： | 刘新喜 彭立凤 |
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| 作者单位： | 河北师范大学 学报编辑部，河北师范大学 化学系 河北 石家庄 050091 河北师范大学 化学系，河北 石家庄 050016，河北 石家庄 050016 |
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| 基金项目： | 河北省自然科学基金(299211) |
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| 摘 要： | 研究了蛋壳作载体,吸附交联法固定化脂肪酶的最佳工艺;C_(酶)=0.12g·g~(-1)cor,t_(吸附)=30min,pH_(交联)=6.2,t_(温度)=室温,得到的最大固定化酶活力为29.41U·g_(car)~(-1);并考察了该固定化酶的稳定性:pH=9.0,t=35℃,交联后的固定化酶重复使用6次后活力仍保贸53％。
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| 关 键 词： | 脂肪酶 载体 蛋壳 吸附交联法 固定化酶 温度稳定性 酸度稳定性 酶活力 |
Immobilization of Lipase Using Egg Shell as Carrier |
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| LIU Xin-xi,PENG Li-feng.Immobilization of Lipase Using Egg Shell as Carrier[J].Journal of Ningxia Teachers College,2000,21(6):21-24. |
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| Authors: | LIU Xin-xi PENG Li-feng |
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| Abstract: | The immobilization of lipase using egg shell as carrier by chemsorption was studied. The absorption conditions were optimized at pH of 6.2,room temperature,30 minutes of the link time.0.12g of free enzyme loaded with Ig of egg shell.The maxium activity obtained on above conditions was 29.41U - g.The stability of it was investigated also.The optimal temperature and pH value was at 35C ,9.0 respectively . The activity of it ratains 53 % after reusing times. . |
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| Keywords: | immobilization lipase carrier |
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