| 美洲大蠊抗肝纤维化活性提取物ML-D中粘多糖的含量测定 |
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| 引用本文： | 李夸巧,李春艳,杨永寿,陈丽娜,肖培云.美洲大蠊抗肝纤维化活性提取物ML-D中粘多糖的含量测定[J].大理师专学报,2013(3):9-12. |
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| 作者姓名： | 李夸巧 李春艳 杨永寿 陈丽娜 肖培云 |
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| 作者单位： | 大理学院药学与化学学院,云南大理671000 |
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| 基金项目： | 国家自然科学基金资助项目（81060329）; 云南省教育厅重点基金资助项目（2011Z053） |
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| 摘 要： | 目的：建立美洲大蠊抗肝纤维化活性提取物ML-D中粘多糖的含量测定方法。方法：采用苯酚-硫酸法测定粘多糖的含量,采用正交试验设计考察ML-D中粘多糖的水解工艺和显色条件,采用单因素试验考察加热显色时间、苯酚和硫酸的用量。结果：ML-D中粘多糖的最佳水解工艺为：盐酸浓度6 mol/L,固液比为1∶1,水解时间1 h;最佳显色条件：苯酚2 mL,硫酸20 mL,加热显色时间10 min。结论：该法操作简便,精密度高,重复性好,可作为美洲大蠊抗肝纤维化活性提取物ML-D中粘多糖含量的测定方法。
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| 关 键 词： | 美洲大蠊 抗肝纤维化 粘多糖 硫酸-苯酚法 含量测定 |
Determination of Glycosaminoglycan in Active Extracts ML-D with Anti-hepatic-fibrosis from Periplaneta americana |
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| LI Kuaqiao,LI Chunyan,YANG Yongshou,CHEN Lina,XIAO Peiyun.Determination of Glycosaminoglycan in Active Extracts ML-D with Anti-hepatic-fibrosis from Periplaneta americana[J].Journal of Dali Teachers College,2013(3):9-12. |
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| Authors: | LI Kuaqiao LI Chunyan YANG Yongshou CHEN Lina XIAO Peiyun |
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| Institution: | ＊ (College of Pharmacy and Chemistry, Dali University, Dali, Yunnan 671000, China) |
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| Abstract: | Objective： To establish a method for determining of glycosaminoglycan in active extracts ML-D with anti-hepatic-fibrosis from Periplaneta americana. Methods： Phenol-vitriol colorimetry was used for determining content of glycosaminoglycan; orthogonal design Lg（ 34） was adopted to investigate hydrolysis process of glycosaminoglycan in ML-D; single factor experiment was used to investigate time of color, dosage of phenol and vitriolic. Results： For the best hydrolysis process of glycosaminoglycan in ML-D, the density of hydrochloric acid is 6 tool/L, Solid-liquid ratio is 1 ： 1, and time of hydrolysis is 1 h. There are the best conditions of color, the dosage of phenol and vitriolic respectively is 2 mL and 20 mL, heating time is 10 min. Conclusion： The method is simple and accurate with reproducibility; it can be used for the determining content of glycosaminoglycan in active extracts ML-D with anti-hepatic-fibrosis from PeriDlaneta americana. |
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| Keywords: | Periplaneta americana anti-hepatic-fibrosis glycosaminoglycan phenol-vitriol colorimetry content determination |
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