| HPLC法测定川滇桤木中绿原酸的含量 |
| |
| 引用本文： | 尹暗梅,杨晓珍,曹艳花,刘婷,肖培云.HPLC法测定川滇桤木中绿原酸的含量[J].大理师专学报,2013(6):20-22. |
| |
| 作者姓名： | 尹暗梅 杨晓珍 曹艳花 刘婷 肖培云 |
| |
| 作者单位： | 大理学院药学与化学学院,云南大理671000 |
| |
| 基金项目： | 大理学院大学生科研基金资助项目（DXSKYYX201318） |
| |
| 摘 要： | 目的：建立HPLC法测定川滇桤木中绿原酸的含量。方法：采用高效液相色谱法，VenusilXBP-C．。色谱柱（150mm×4．6mm，5μm），流动相为乙腈-0．4％磷酸溶液（8：92），流速为1．0mL／min，检测波长为324nm，柱温25℃。结果：绿原酸在0．1088μg-1．088μg范围内线性关系良好，r=0．9999，平均加样回收率为98．98％，RSD=0．77％（n=6）。结论：本法操作简便、快速、稳定性高、重现性好，可用于川滇桤木中绿原酸含量的测定。
|
| 关 键 词： | 川滇桤木 绿原酸 高效液相色谱 |
Determination of Chlorogenic Acid in Alnus ferdinandi-coburgii by HPLC |
| |
| YIN Lamei,YANG Xiaozhen,CAO Yanhua,LIU Ting,XIAO Peiyun.Determination of Chlorogenic Acid in Alnus ferdinandi-coburgii by HPLC[J].Journal of Dali Teachers College,2013(6):20-22. |
| |
| Authors: | YIN Lamei YANG Xiaozhen CAO Yanhua LIU Ting XIAO Peiyun |
| |
| Institution: | ＊ (College of Pharmacy and Chemistry, Dali University, Dali, Yunnan 671000, China) |
| |
| Abstract: | Objective： To establish an HPLC method for the determination of chlorogenic acid in Alnus ferdinandi-coburgii. Methods： We applied highly effective liquid phase chromatography, with Venusil X BP-C18 column （150 mm×4.6 mm,5 μm） and mobile phase consisted of acetonitrile-0.4% phosphoric acid solution （8：92） at a flow rate of 1.0 mL/min. The detection wavelength was set at 324 nm, and column temperature was 25 ℃. Results： There was good linearity in the range of 0.108 8-1.088 μg （r：0.999 9） of chlorogenic acid. The average recovery was 98.98%, RSD was 0.77%（n=6）. Conclusion： The method above was simple, sensitive, accurate, reproducible, and could be applied in the measurement of chlorogenic acid in A lnus ferdinandi-coburgii. |
| |
| Keywords: | Alnus ferdinandi-coburgii chlorogenic acid HPLC |
| 本文献已被 维普 等数据库收录！ |
|
