| 中药王不留行的组织培养技术初探 |
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| 引用本文： | 贲爱玲,蔡小宁,任源浩,汪晓婷.中药王不留行的组织培养技术初探[J].南京晓庄学院学报,2006,22(6):53-56. |
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| 作者姓名： | 贲爱玲 蔡小宁 任源浩 汪晓婷 |
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| 作者单位： | 1. 南京晓庄学院,生命科学系,江苏,南京,210017
2. 南京大学,生命科学学院,江苏,南京,210093 |
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| 基金项目： | 南京晓庄学院科技处重点项目资助(No.4041004) |
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| 摘 要： | 选取王不留行饱满种子培养无菌苗,用无菌苗的茎和叶作为外植体,分别接种MS基本培养基外加激素NAA和6-BA诱导愈伤组织和不定芽,使用1/2MS培养基,附加IBA或NAA进行不定芽的生根实验,最后炼苗和移栽.实验结果表明王不留行的最优组织培养方案:种子用0.1%升汞消毒8m in,无菌湿脱脂棉催芽,茎和叶作为外植体,使用MS 0.1mg/L NAA 0.1mg/L 6-BA诱导愈伤组织和不定芽,1/2MS 0.15mg/L NAA诱导植株生根.
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| 关 键 词： | 王不留行 组织培养 |
| 文章编号： | 1009-7902（2006）06-0053-04 |
| 收稿时间： | 2006-04-24 |
| 修稿时间： | 2006年4月24日 |
The Tissue Culture Procedure in the Chinese Traditional Medicine of Vacaria Segetalis (Neck) Carcke |
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| BEN Ai-ling,CAI Xiao-ning,REN Yuan-hao,WANG Xiao-ting.The Tissue Culture Procedure in the Chinese Traditional Medicine of Vacaria Segetalis (Neck) Carcke[J].Journal of Nanjing Xiaozhuang College,2006,22(6):53-56. |
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| Authors: | BEN Ai-ling CAI Xiao-ning REN Yuan-hao WANG Xiao-ting |
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| Abstract: | An experiment was conducted,which germinated sterile plantlet by the seeds,induced callus and adventitious shoots in MS media supplemented with 6-BA and NAA but induced roots in 1/2 MS media supplemented with IBA and NAA,and finally transplanted plantlet in Vacaria Segetalis(Neck) Carcke.The result showed that the fine tissue culture procedure was that 0.1% HgCl_2 was 8 min,the bud was germinated above the wet absorbent cotton under sterile condition,callus and adventitious shoots were produced on the stem and leaf piece in the MS media supplemented with 0.1mg/L NAA 0.1mg/L 6-BA,and the new propagule was easy to root in the 1/2 MS media supplemented with 0.15mg/L NAA. |
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| Keywords: | Vacaria Segetalis(Neck) Carcke tissue culture |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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