| 半夏生物碱提取方法及抗氧化性研究 |
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| 引用本文： | 张楠,郭春延,薛晶晶,楚建周,姚晓芹.半夏生物碱提取方法及抗氧化性研究[J].实验技术与管理,2019(8):61-64. |
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| 作者姓名： | 张楠 郭春延 薛晶晶 楚建周 姚晓芹 |
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| 作者单位： | 河北大学生命科学学院 |
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| 基金项目： | 国家自然科学基金资助项目(31300321);河北省自然科学基金项目(C2012201080) |
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| 摘 要： | 研究了不同提取方法对半夏生物碱含量的影响,并对半夏生物碱的抗氧化性进行分析。结果表明,最佳提取方法为热浸,最佳提取时间为1 h,最佳温度分为35~40℃,稳定性、精密度和回收率分别为0.341%、0.052%和102.56%。半夏DPPH自由基清除活性显著高于超氧自由基清除活性和羟自由基清除活性。该提取方法简单、省时、稳定,该方法可以应用于大学生综合性实验中。
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| 关 键 词： | 半夏 生物碱 提取方法 抗氧化活性 |
Study on extraction methods and antioxidant activity of alkaloids from Pinellia ternata |
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| ZHANG Nan,GUO Chunyan,XUE Jingjing,CHU Jianzhou,YAO Xiaoqin.Study on extraction methods and antioxidant activity of alkaloids from Pinellia ternata[J].Experimental Technology and Management,2019(8):61-64. |
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| Authors: | ZHANG Nan GUO Chunyan XUE Jingjing CHU Jianzhou YAO Xiaoqin |
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| Institution: | (College of Life Science, Hebei University, Baoding 071002, China) |
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| Abstract: | The effects of different extraction methods on the alkaloids content of Pinellia ternata are studied, and the antioxidant activity of the alkaloids is analyzed. The results show that the optimum extraction method is hot soaking, the optimum extraction time is 1 h, and the optimum temperature is from 35 °C to 40 °C. The stability, precision and recovery are 0.341%, 0.052% and 102.56% respectively. DPPH radical scavenging activity of Pinellia ternata is significantly higher than superoxide radical scavenging activity and hydroxyl radical scavenging activity. This method is simple, time-saving and stable, so it can be applied to comprehensive experiments for college students. |
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| Keywords: | Pinellia ternata alkaloids extraction methods antioxidant activity |
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