| 防污损涂料添加剂Irgarol-1051降解产物对H4IIE鼠肝癌细胞的基因影响(英文) |
| |
| 引用本文： | 许妍,林家豪,林汉华.防污损涂料添加剂Irgarol-1051降解产物对H4IIE鼠肝癌细胞的基因影响(英文)[J].东南大学学报,2014(4):506-513. |
| |
| 作者姓名： | 许妍 林家豪 林汉华 |
| |
| 作者单位： | 1. 东南大学土木工程学院，南京210096; 香港城市大学生物与化学系，中国香港
2. 香港城市大学生物与化学系，中国香港 |
| |
| 基金项目： | The Research Grants Council of Hong Kong SAR China No．CityU 1445／05M the National Natural Science Founda-tion of China No．41301546． |
| |
| 摘 要： | 为研究海洋防污损涂料添加剂Irogarol-1051的降解产物M2的基因毒性,应用微阵列技术,选取Affymetrix公司鼠基因组230 2.0基因芯片检测30μmol/L M2暴露下的鼠肝癌细胞基因表达变化.实验结果显示,96 h的M2暴露导致了38个基因在全部4组可能的对照/暴露中均发生显著变化(p0.002 5),其中只有Accn5基因研究较为透彻,该基因表达的抑制可能影响上皮钠通道的功能.此外,分别有10和82个功能注释基因在至少一组对照/暴露组中上调和下调.M2诱导的基因主要和细胞核(细胞成分)相关.M2抑制的基因则主要影响生物过程中的G蛋白偶联信号通路功能和细胞成分中的细胞膜内整合功能.
|
| 关 键 词： | Irgarol- 降解产物M 微阵列 基因表达 基因毒性 |
Gene expression profile in H4 IIE rat hepatoma cells exposed to an antifouling booster biocide Irgarol-1051 degradation product |
| |
| Xu Yan,Lam Ka-Ho,Lam Michael Hon-Wah.Gene expression profile in H4 IIE rat hepatoma cells exposed to an antifouling booster biocide Irgarol-1051 degradation product[J].Journal of Southeast University(English Edition),2014(4):506-513. |
| |
| Authors: | Xu Yan Lam Ka-Ho Lam Michael Hon-Wah |
| |
| Institution: | Xu Yan, Lam Ka-Ho, Lam Michael Hon-Wah ( 1 School of Civil Engineering, Southeast University, Nanjing 210096, China) (2Department of Biology and Chemistry, City Uifiversity of Hong Kong, Hong Kong, China) |
| |
| Abstract: | To better understand the toxicity of an antifouling booster biocide Irgarol-1051 degradation product M2 3-4-tert-butylamino-6-methylthiol-s-triazin-2-ylamino pro-pionaldehyde this study utilized a DNA microarray technique to explore the genotoxicity of M2. The Affymetrix Inc. rat genome 230 2.0 GeneChip was employed to examine alterations in gene regulation in rat hepatoma cells exposed to 30 μmol/L of M2 for 96 h.The results showed that 38 genes were significantly p＆lt;0.002 5 altered by M2 at two-fold changes in all the four possible control/exposure comparisons. Accn5 was the only well described gene consistently being suppressed which likely altered the epithelial sodium channel ENaC .10 and 82 annotated genes were up-and down-regulated in at least one of the control/exposure comparisons respectively. The induced genes were mainly involved in the nucleus belonging to the cellular component. The largest categories of suppression concerned G-protein coupled receptor protein signaling pathways belonging to the biological process and integral to membranes belonging to the cellular component. |
| |
| Keywords: | Irgarol-1051 degradation product M2 microarray gene expression genotoxicity |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
