Anti-angiogenesis effect of generation 4 polyamidoamine/vascular endothelial growth factor antisense oligodeoxynucleotide on breast cancer in vitro |
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Authors: | Shan-zhi Gu Xin-han Zhao Ling-xiao Zhang Li Li Zhi-yu Wang Min Meng Gai-li An |
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Institution: | (1) MOE Key Laboratory of Environment and Genes Related to Diseases, Xi’an Jiaotong University, Xi’an, 710061, China;(2) Department of Medical Oncology, the First Affiliated Hospital, College of Medicine, Xi’an Jiaotong University, Xi’an, 710061, China |
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Abstract: | Objective: To study the effects of the generation 4 polyamidoamine/vascular endothelial growth factor antisense oligodeoxynucleotide (G4PAMAMNEGFASODN) compound on the expressions of vascular endothelial growth factor (VEGF) and its mRNA of breast cancer cells and on the inhibition of vascular endothelial cells. Methods: We examined the morphology of G4PAMAM/VEGFASODN compound and its pH stability, in vitro transfection efficiency and toxicity, and the expressions of VEGF and its mRNA. Methyl thiazolyl tetrazolium assay was used to detect the inhibitory function of the compound on vascular endothelial cells. Results: The compound was about 10 nm in diameter and was homogeneously netlike. From pH 5 to 10, it showed quite a buffered ability. The 48-h transfection rate in the charge ratio of 1:40 was 98.76%, significantly higher than that of the liposome group (P<0.05). None of the transfection products showed obvious toxicity on the cells. The expressions of both VEGF protein and its mRNA after G4PAMAM/VEGFASODN transfection decreased markedly. Conclusion: With a low toxicity, high safety, and high transfection rate, G4PAMAMNEGFASODN could be a promising gene vector. Specifically, it inhibits VEGF gene expression efficiently, laying a basis for further in vivo animal studies. |
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Keywords: | Breast cancer Vascular endothelial growth factor (VEGF) VEGF Mrna Generation 4 polyamidoamine (G4PAMAM) Generation 4 polyamidoamine/vascular endothelial growth factor antisense oligodeoxynucleotide (G4PAMAM/VEGFASODN) Vascular endothelial cell |
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