Inducible nitric oxide synthase expression is related to angiogenesis,bcl-2 and cell proliferation in hepatocellular carcinoma |
| |
| Authors: | Peng?Jia-ping Email author" target="_blank">Zheng?Shu?Email author Xiao?Zuo-xiang Zhang?Su-zhan |
| |
| Institution: | (1) Cancer Institute, College of Medicine, Zhejiang University, 310009 Hangzhou, China |
| |
| Abstract: | In this study, we examined the expression of inducible nitric oxide synthase (iNOS) and vascular endothelial growth factor
(VEGF) by immunohistochemical staining in 76 tissue sections collected from hepatocellular carcinoma (HCC) patients undergoing
hepatectomy. Microvascular density (MVD) was determined by counting endothelial cells immunostained using anti-CD34 antibody.
We performed DNA-flow cytometric analyses to elucidate the impact of iNOS and VEGF expression on the cell cycle of HCC. Most
of the HCC cells that invaded stroma were markedly immunostained by iNOS antibody. The iNOS stain intensity of the liver tissue
close to the tumor edge was stronger than that of HCC tissue, and the strongest was the hepatocytes closer to the tumor tissue.
However, iNOS expression in 10 normal hepatic samples was undetectable. VEGF positive expression ratio was 84.8% in iNOS positive
expression cases, and the ratio was 35.3% in negative cases. There was significant correlation (P=0.000) between iNOS and VEGF expression. Moreover, iNOS expression was significantly associated with bcl-2 and MVD, but without
p53 expression. DNA-flow cytometric analyses showed that combined expression of iNOS and VEGF had significant impact on the
cell cycle in HCC. PI (Proliferating Index) and SPF (S-phase fraction) in the combined positive expression of iNOS and VEGF
group was significantly higher than that in the combined negative group. The present findings suggested that iNOS expression
was significantly associated with angiogenesis, bcl-2 and cell proliferation of HCC.
Project supported in part by the National Ninth-Five-Years Project Fund (No. 96909121), China |
| |
| Keywords: | Hepatocellular carcinoma Nitric oxide synthase Angiogenesis Bcl-2 Flow cytometric analyse |
| 本文献已被 万方数据 SpringerLink 等数据库收录！ |
|
