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Relationships between endothelial nitric oxide synthase gene polymorphisms and osteoporosis in postmenopausal women |
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| Authors: | Shun-zhi Liu Hong Yan Wei-kun Hou Peng Xu Juan Tian Li-fang Tian Bo-feng Zhu Jie Ma She-min Lu |
| Institution: | 1. Department of Public Health, School of Medicine, Xi’an Jiaotong University, Xi’an, 710061, China 2. Key Laboratory of Environment and Genes Related to Diseases of Ministry of Education, Xi’an Jiaotong University, Xi’an, 710061, China 3. Department of Genetics and Molecular Biology, School of Medicine, Xi’an Jiaotong University, Xi’an, 710061, China 4. Department of Bone and Joint Diseases of Xi’an Red Cross Hospital, Xi’an, 710054, China 5. Department of Forensic Medicine, School of Medicine, Xi’an Jiaotong University, Xi’an, 710061, China |
| Abstract: | Objective To investigate the relationships between endothelial nitric oxide synthases (eNOS) G894T and 27 bp-variable number tandem repeat (VNTR) gene polymorphisms and osteoporosis in the postmenopausal women of Chinese Han nationality. Methods In the present study, 281 postmenopausal women from Xi’an urban area in West China were recruited, and divided into osteoporosis, osteopenia, and normal groups according to the diagnostic criteria of osteoporosis proposed by World Health Organization (WHO). The bone mineral density (BMD) values of lumbar vertebrae and left hips were determined by QDR-2000 dual energy X-ray absorptiometry. Blood samples were tested for plasma biochemical indicators including testosterone, estradiol, calcitonin, osteocalcin, and procollagen type I amino-terminal propeptide by enzyme-linked immunosorbent assay (ELISA), tartrate-resistant acid phosphatase by spectrophotometric method, and the content of nitric oxide by Griess method. Genome DNA was extracted from whole blood, and G894T polymorphism of eNOS gene was analyzed by using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method and 27 bp-VNTR polymorphism of eNOS gene was genotyped by PCR method. Then the relationships between genotypes and biochemical indicators, genotypes and osteoporosis, and haplotypes and osteoporosis were analyzed. Results The average BMD values of the femoral neck, ward’s triangle and lumbar vertebrae 1∼4 (L1∼L4) in the subjects with T/T genotype in eNOS G894T locus were significantly higher than those in the subjects with G/T and G/G genotypes (P<0.05). The average BMD of the femoral neck in the subjects with a/a genotype of eNOS 27 bp-VNTR locus was evidently higher than that in the subjects with b/b genotype (P<0.05). The plasma testosterone and osteocalcin concentrations in the subjects of eNOS G894T G/T genotype were evidently higher than those in the subjects of other genotypes (P<0.05); the plasma estradiol concentration in the subjects of eNOS 27 bp-VNTR a/a genotype was obviously higher than that in the subjects of b/b genotype (P<0.01). eNOS G/G homozygous frequencies in osteoporosis women, osteopenia women, and normal women were 85.37%, 76.38%, and 83.87%, respectively (P>0.05). 0% osteoporosis woman, 0.79% osteopenia women, and 3.23% normal women were eNOS a/a homozygous (P<0.05). The frequencies of eNOS 27 bp-VNTR a allele were 5.33% in the osteoporosis group, 10.24% in the osteopenia group, and 16.13% in the normal group (P<0.05, odds ratio (OR)=0.29, 95% confidence interval (CI)=0.11∼0.77), suggesting that a/a genotype and a allele might have protective effects on osteoporosis. The haplotype analysis showed that G-b was 87.7% (214/244) in the osteoporosis group (P<0.05, OR=2.48, 95% CI=1.18∼5.18). G-a was 5.3% (13/244) in the osteoporosis group (P<0.05, OR=0.29, 95% CI=0.11∼0.77). G-b was a risk factor for osteoporosis, and G-a a protective factor. Conclusion eNOS G894T G/T genotype influenced the plasma testosterone and osteocalcin concentrations, and T/T genotype influenced BMD. eNOS 27 bp-VNTR a/a genotype increased plasma estradiol concentration to have a protective effect on osteoporosis. Project supported by the National Natural Science Foundation of China (Nos. 30630058 and 30571725), the Xi’an Municipal Science and Technology Research Project Fund (No. GG06152), and the Shanxi Provincial Science and Technology Research and Development Project Fund (No. 2007K14-01), China |
| Keywords: | Postmenopausal women Osteoporosis Endothelial nitric oxide synthase Gene polymorphisms Bone mineral density |
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