| 铁观音茶和茶茎原花青素含量的测定 |
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| 引用本文： | 吴艳艳,侯冬岩,回瑞华.铁观音茶和茶茎原花青素含量的测定[J].鞍山师范学院学报,2012,14(2):30-33. |
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| 作者姓名： | 吴艳艳 侯冬岩 回瑞华 |
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| 作者单位： | 1. 辽宁师范大学化学化工学院,辽宁大连116029／鞍山师范学院化学与生命科学学院,辽宁鞍山114007
2. 鞍山师范学院化学与生命科学学院,辽宁鞍山114007／辽宁师范大学化学化工学院,辽宁大连116029 |
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| 摘 要： | 对铁观音茶和茶茎原花青素的含量进行测定.采用超声提取法,90%乙醇做提取剂,用1%的香草醛-盐酸溶液作为显色剂,对样品中的原花青素含量进行测定.用原花青素标准品得到线性回归方程为:A=8.301 3×C-0.047 3,相关系数R=0.999 6,原花青素的浓度在0.020～0.120 mg/mL范围内,吸光度A与浓度C呈良好线性关系.方法的加标回收率为96.5%～101.5%,变异系数为0.01%,该方法的准确度与精密度均较高,可按标准曲线法进行定量分析.
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| 关 键 词： | 安溪铁观音茶 茶茎 原花青素 紫外分光光度法 |
Tieguanyin Tea and Tea Stalk the Proanthocyanidins Content Determination |
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| WU Yan-yan,HOU Dong-yan,HUI Rui-hua.Tieguanyin Tea and Tea Stalk the Proanthocyanidins Content Determination[J].Journal of Anshan Teachers College,2012,14(2):30-33. |
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| Authors: | WU Yan-yan HOU Dong-yan HUI Rui-hua |
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| Institution: | 1(1.College of Chemistry and Chemical Engineering,Liaoning Normal University,Dalian Liaoning 116029,China; 2.School of Chemistry and Life Science,Anshan Normal University,Anshan Liaoning 114007,China) |
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| Abstract: | In this paper,Tieguanyin tea leaf and tea stems the proanthocyanidins content determination.Using ultrasonic extraction method,90% ethanol as extraction agent,with 1% vanillin HCl solution as the chromogenic agent,on the proanthocyanidins content determination.With proanthocyanidins standard linear regression equation:A=8.301 3 x C-0.047 3,correlation coefficient R=0.999 6,proanthocyanidin concentration in the 0.020～0.120 mg/mL range,the absorbance of A and concentration of C showed good linear relationship.Method of standard addition recovery rate was 96.5%～101.5%,the variation coefficient was 0.01%,the accuracy and precision of this method are high.According to the standard curve method in quantitative analysis. |
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| Keywords: | Tieguanyin tea leaf Tea stems Proanthocyanidins UV Spectrophotometry |
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