Binding interactions of pefloxacin mesylate with bovine lactoferrin and human serum albumin |
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| Authors: | Ji-cai Fan Xiang Chen Yun Wang Cheng-ping Fan Zhi-cai Shang |
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| Institution: | (1) Department of Chemistry, Zhejiang University, Hangzhou, 310027, China |
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| Abstract: | The binding of pefloxacin mesylate (PFLX) to bovine lactoferrin (BLf) and human serum albumin (HSA) in dilute aqueous solution was studied using fluorescence spectra and absorbance spectra. The binding constant K and the binding sites n were obtained by fluorescence quenching method. The binding distance r and energy-transfer efficiency E between pefloxacin mesylate and bovine lactoferrin as well as human serum albumin were also obtained according to the mechanism of Förster-type dipole-dipole nonradiative energy-transfer. The effects of pefloxacin mesylate on the conformations of bovine lactoferrin and human serum albumin were also analyzed using synchronous fluorescence spectroscopy. |
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| Keywords: | Pefloxacin mesylate (PFLX) Bovine lactoferrin (BLf) Human serum albumin (HSA) Fluorescence spectra Energy-transfer efficiency |
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