| 18-26S rDNA在4种重楼属植物中的定位 |
| |
| 引用本文： | 王丽,李云飞,唐荣华,顾志建.18-26S rDNA在4种重楼属植物中的定位[J].中国科学院研究生院学报,2004,42(5):419-426. |
| |
| 作者姓名： | 王丽 李云飞 唐荣华 顾志建 |
| |
| 作者单位： | 四川大学生命科学学院 |
| |
| 摘 要： | 为探讨rDNA在重楼属Paris L.中的分布规律，利用荧光原位杂交（FISH）对4种重楼属植物
的18-26S rDNA进行了定位。所有植物均为二倍体，基因组由A、B、C、D和
E5条染色体构成。（1）滇重楼P.polyphylla var.yunnanensis:2n=10=6m+4t，C和D染色体的
短臂上各有1个18-26S rDNA位点;（2）长柱重楼P.forrestii:2n=10=6m+4t，B染色体的长臂
、C和D染色体的短臂上各有1个位点;（3）五指莲P.axialis:2n=10=6m（2sat）+4t（2sat）
+1-2B，C和D染色体的短臂上各有1个位点;在有1个B染色体的细胞中，B染色体没有信号点，
而有2个B染色体的细胞中，只有1个B染色体上有信号点，表明B染色体上有基因存在且其分裂
不均等;（4）大理重楼P.daliensis:2n=10=4m+2sm+2st+2t，C染色体的短臂上有1个位点。1
8-26S rDNA位点不仅出现在染色体的次缢痕上，也出现在非次缢痕位点。另外，4个种中C染
色体短臂末端均有18-26S rDNA。
|
| 关 键 词： | 重楼属 荧光原位杂交 18-26S rDNA B染色体 |
Mapping of18-26S rDNA loci in four species of the genus Paris by fluorescence in situ hybridization（FISH） |
| |
| WANG Li,LI Yun-Fei,TANG Rong-Hua,GU Zhi-Jian.Mapping of18-26S rDNA loci in four species of the genus Paris by fluorescence in situ hybridization（FISH）[J].Journal of the Graduate School of the Chinese Academy of Sciences,2004,42(5):419-426. |
| |
| Authors: | WANG Li LI Yun-Fei TANG Rong-Hua GU Zhi-Jian |
| |
| Abstract: | 18-26S rDNA loci were mapped on chromosomes in four species of Par
is，and the num-ber and position of rDNA sites in these species were compared f
or analysis of the distribution of the sites. All the plants were diploids，and t
he genome consisted of five chromosomes，A，B，C，D and E. （1）P. polyphylla var.
yunnanensis，2n=10=6m+4t. Two18-26S rDNA loci were de-tected on the short arms o
f C and D chromosomes;（2）P. forrestii，2n=10=6m+4t. One locus was detected on th
e long arm of B chromosome，and also two loci on the short arms of C and D chro-
mosomes;（3）P. axialis. 2n=10=6m（2sat）+4t（2sat）+1-2B. Two loci were detected o
n the short arms of C and D chromosomes. One locus was detected in the cell with t
wo B-chromosomes（B），but none was detected in that with only one B chromosome，
indicating that rRNA gene existed on B chromsome，and an unequal division occurr
ed during mitotic cycle of B-chromosomes. （4）P. daliensis，2n=10=4m+2sm+2st+2t. O
ne locus was detected on the short arm of D chromo-some. The signals of18-26S rD
NA appeared not only in the second constriction but also in the other regions of
chromosome. It is noteworthy that one locus was detected in the terminal region o
n the short arm of C chromosome in all the four species studied. |
| |
| Keywords: | Paris fluorescence in situ hybridization（FISH） 18-26S rDNA B c |
|
| 点击此处可从《中国科学院研究生院学报》浏览原始摘要信息 |
| 点击此处可从《中国科学院研究生院学报》下载免费的PDF全文 |
